重组恒河猴FCGR3蛋白
- 蛋白种属:恒河猴
- 表达宿主:人胚胎肾细胞(HEK293)
- Unitprot ID:F5A869
产品详情
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产品名称
重组恒河猴FCGR3蛋白
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蛋白种属
恒河猴
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表达宿主
人胚胎肾细胞(HEK293)
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Unitprot ID
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克隆构建
A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1-Gly 206) was fused with a polyhistidine tag at the C-terminus.
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蛋白纯度
> 95 % as determined by SDS-PAGE
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蛋白活性
Measured by its binding ability in a functional ELISA.
Immobilized Rhesus FCGR3-His (cat:504599) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:500583). The EC50 of human IgG1 (Fc) (Cat:500583) is 0.35-0.81 μg/ml.
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分子量
The recombinant rhesus CD16 consists of 201 amino acids and has a calculated molecular mass of 22.7 kDa.
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蛋白别名
FCGAMMARIV; FCR-10; FCGRIV; FCRIII; FCRIIIA; CD16; FC GAMMA RIII; FCG3; CD16-2; FCGR4; RIII; IGFR3; FCGRIII; FCGR3A; FCRIIIB; FC Γ FCGR3
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蛋白描述
Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
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发货形式
Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!