RP-832c 多肽

产品详情

  • 产品名称
    RP-832c 多肽
  • 序列
    H-RWKFGGFKWR-OH
  • 三字母序列
    H-Arg-Trp-Lys-Phe-Gly-Gly-Phe-Lys-Trp-Arg-OH
  • 长度 (aa)
    10
  • 多肽纯度 (HPLC)
    99.62
  • 分子式
    C68H94N20O11
  • 分子量
    1367.6
  • 来源
    固相合成
  • 描述

    Peptide RP-832c is a novel synthetic analogue of a naturally occurring Host Defense Peptide (HDP), designed to target the CD206 receptor on M2-polarized macrophages. This peptide plays a crucial role in mediating innate immune responses and has shown promising results in reducing pro-fibrotic cytokines in various models of lung fibrosis.

    Activated M2-polarized macrophages are known drivers of pulmonary fibrosis in conditions like Idiopathic Pulmonary Fibrosis (IPF). RP-832c binds selectively to the CD206 receptor on these macrophages, leading to a time-dependent decrease in CD206 expression and an increase in M1-macrophage markers such as TNF-α. This action mechanism suggests that RP-832c can modulate macrophage activation states, shifting them from a pro-fibrotic M2-like phenotype to a potentially less harmful M1-like phenotype.

    The efficacy of RP-832c has been tested in vitro using bone marrow-derived macrophages (BMDM) and in vivo using a murine model of bleomycin (BLM)-induced early-stage pulmonary fibrosis. In these models, RP-832c demonstrated a significant reduction in fibrosis in a dose-dependent manner, decreased expression of pro-fibrotic markers like CD206, TGF-β1, α-SMA, and inflammatory cytokines such as TNF-α, IL-6, IL-10, IFN-γ, CXCL1/2. Moreover, RP-832c's effects on reducing lung fibrosis were comparable or even superior to FDA-approved drugs like Nintedanib and Pirfenidone, without exhibiting any significant toxicity at the tested doses.

    RP-832c represents a potential new avenue for treating pulmonary fibrosis, targeting the activated M2-like macrophages that are central to the disease's progression. Its ability to modify macrophage phenotype and reduce fibrosis without significant toxicity highlights its therapeutic potential for patients with pulmonary fibrosis

  • 储存指南
    一般情况下, RP-832c 多肽 粉末应该保存在-20甚至-70摄氏度. RP-832c 多肽 溶解后应该-70摄氏度分装保存,避免反复冻融. 了解更多细节,请查阅手册:多肽溶解及保存指南
  • 参考文献
    • Ghebremedhin A, Salam AB, Adu-Addai B, Noonan S, Stratton R, Ahmed MSU, Khantwal C, Martin GR, Lin H, Andrews C, Karanam B, Rudloff U, Lopez H, Jaynes J, Yates C. A Novel CD206 Targeting Peptide Inhibits Bleomycin-Induced Pulmonary Fibrosis in Mice. Cells. 2023 Apr 26;12(9):1254. doi: 10.3390/cells12091254. PMID: 37174654; PMCID: PMC10177262.
  • 关于三氟乙酸盐

    三氟乙酸(TFA)是一种强酸,常用于从固相树脂中裂解合成的多肽,也可用于提高多肽纯化步骤中的HPLC性能。默认情况下,定制的多肽是以冻干的TFA盐的形式交付的,其中TFA的含量可能高达10-45%。

    定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如,nM浓度的TFA已被证明会干扰细胞实验,在某些情况下会抑制细胞增殖,而在其他情况下会增加细胞活力。

    TFA去除服务推荐用于:

    • 将用于细胞试验的多肽
    • 将被用作原料药或制成品的多肽
    • 含有大量碱性残基的亲水多肽

  • 关于多肽净含量

    干品多肽的重量中不仅仅包含多肽,还包含有一些非肽的组份,如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比,这个百分比的数值范围很大,可能从50%到90%,取决于纯度、序列以及合成和纯化的方法,不要将肽的净含量和肽的纯度混为一谈,他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比,而肽的净含量是指样品中肽类物质相对于总物质所占的百分比,肽的净含量通常是氨基酸组分分析或紫外分光法测定的,这个信息主要是在一些对肽的浓度很敏感的实验中,对计算肽的浓度是很重要的。

多肽合成服务: 纽普生物(NovoPro)提供常规多肽合成、糖修饰多肽合成、稳定同位素标记的多肽合成、磷酸化多肽合成、荧光标记多肽、多肽的BSA/KLH/OVA等载体蛋白的偶联等服务

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