在线密码子优化工具(ExpOptimizer)
ExpOptimizer密码子优化工具用于优化在主流宿主中表达重组基因的密码子。其优化的参数包含转录和翻译两个过程多达十几个关键参数*.
请输入基因的编码区(CDS),长度必须必须是3的倍数;如果输入的是蛋白质序列,请先选择序列类型,然后再输入。
0. 序列类型:
1. 序列 (请输入序列,非FASTA格式):
全长:0
优化参数(部分)
- 密码子使用偏好性
- 5' 区域优化(翻译起始效率)
- DNA 重复序列
- mRNA 二级结构
- GC 含量
- SD 序列
- 排除指定的限制性内切酶位点
参考文献
- Villalobos A, Ness JE, Gustafsson C, Minshull J, Govindarajan S. Gene Designer: a synthetic biology tool for constructing artificial DNA segments. BMC Bioinformatics. 2006
- Lithwick G, Margalit H: Hierarchy of sequence-dependent features associated with prokaryotic translation. Genome Res 2003, 13:2665-2673.
- Jin H, Zhao Q, Gonzalez de Valdivia E, Ardell DH, Stenström M, Isaksson LA: Influences on gene expression in vivo by a Shine-Dalgarno sequence. Mol Microbiol 2006, 60:480-92.
- Laursen BS, Sørensen HP, Mortensen KK, Sperling-Petersen HU: Initiation of Protein Synthesis in Bacteria. Microbiol Mol Biol Rev 2005, 69:101-123.
- Stenstrom CM, Holmgren E, Isaksson LA: Cooperative effects by the initiation codon and its flanking regions on translation initiation. Gene 2001, 273:259-265.