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在线密码子优化工具（ExpOptimizer）

ExpOptimizer密码子优化工具用于优化在主流宿主中表达重组基因的密码子。其优化的参数包含转录和翻译两个过程多达十几个关键参数*. 根据算法，每次计算会随机采样，返回局部最优结果，因此每次提交返回的结果都会有差异。

请输入基因的编码区（CDS），长度必须必须是3的倍数；如果输入的是蛋白质序列，请先选择序列类型，然后再输入。

0. 序列类型:

1. 序列 (请输入序列，非FASTA格式):

全长:0

2. 表达宿主:

3. 选择需要排除的限制性内切酶位点:



优化参数（部分）

  • 密码子使用偏好性
  • 5' 区域优化（翻译起始效率）
  • DNA 重复序列
  • mRNA 二级结构
  • GC 含量
  • SD 序列
  • 排除指定的限制性内切酶位点

近期引用该工具论文

  • Zaib S, Rana N, Areeba, Hussain N, Alrbyawi H, Dera AA, Khan I, Khalid M, Khan A, Al-Harrasi A. Designing multi-epitope monkeypox virus-specific vaccine using immunoinformatics approach. J Infect Public Health. 2023 Jan;16(1):107-116.
  • Yufeng Xie, Lijun Qin, Degang Zhao, Preparation of gutta-percha through fermentative degradation of Eucommia ulmoides Oliver leaves by recombinant yeast expressing cellulases from Trichoderma reesei, Industrial Crops and Products, Volume 192, 2023, 115830, ISSN 0926-6690
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  • Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan GA, Yang X. Split selectable marker systems utilizing inteins facilitate gene stacking in plants. Commun Biol. 2023 May 26;6(1):567.
  • Yin Z, Li M, Niu C, Yu M, Xie X, Haimiti G, Guo W, Shi J, He Y, Ding J, Zhang F. Design of multi-epitope vaccine candidate against Brucella type IV secretion system (T4SS). PLoS One. 2023 Aug 10;18(8):e0286358.
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  • Contreras MA, Serrano-Rivero Y, González-Pose A, Salazar-Uribe J, Rubio-Carrasquilla M, Soares-Alves M, Parra NC, Camacho-Casanova F, Sánchez-Ramos O, Moreno E. Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy. Molecules. 2023 Apr 25;28(9):3708.
  • Afshari E, Cohan RA, Sotoodehnejadnematalahi F, Mousavi SF. In-silico design and evaluation of an epitope-based serotype-independent promising vaccine candidate for highly cross-reactive regions of pneumococcal surface protein A. J Transl Med. 2023 Jan 10;21(1):13.
  • Cordes MHJ, Sundman AK, Fox HC, Binford GJ. Protein salvage and repurposing in evolution: Phospholipase D toxins are stabilized by a remodeled scrap of a membrane association domain. Protein Sci. 2023 Jul;32(7):e4701.
  • Zhang C, Li M, Yu T. Bioinformatics analysis of Muscovy duck parvovirus REP and VP1 proteins. J Biomol Struct Dyn. 2023 Sep-Oct;41(15):7174-7189.
  • Lou X, Yao S, Chen P, Wang D, Agassin RH, Hou Y, Zhang C, Ji K. Transcriptome Identification of R2R3-MYB Gene Family Members in Pinus massoniana and PmMYB4 Response to Drought Stress. Forests. 2023; 14(2):410.
  • Lin BC, Kaissarian NM, Kimchi-Sarfaty C. Implementing computational methods in tandem with synonymous gene recoding for therapeutic development. Trends Pharmacol Sci. 2023 Feb;44(2):73-84.