本工具用于蛋白跨膜结构预测，统一支持跨膜 α 螺旋与 β-桶体系，不限物种来源。输入支持多条 FASTA 序列，适用于候选蛋白的批量初筛、膜蛋白表达方案评估、以及信号肽与跨膜区联合判断场景。
模型输出包含全局类别判定、残基层级拓扑标签、跨膜区段边界与信号肽切割位点信息。请注意：该工具用于拓扑结构判定，不直接用于亚细胞定位结论。
1. 蛋白质序列（支持10条 FASTA）:
已解析序列数: 0，总残基数: 0
标签说明（残基层级）
- B：Beta Strand (TM)，跨膜 β 折叠链
- I：Inside (Cytoplasm)，膜内侧/胞质侧
- O：Outside (Extracellular)，膜外侧/胞外侧
- M：Alpha Helix (TM)，跨膜 α 螺旋
- P：Periplasm，周质空间区域
- S：Signal Peptide，信号肽区域
模型性能指标
[k-fold Per-Class Performance (Residue Level)]
Class | Support | Precision | Recall | F1 | MCC
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B | 1458 | 0.8206 | 0.8409 | 0.8306 | 0.8291
I | 108316 | 0.9876 | 0.9971 | 0.9924 | 0.9765
M | 5651 | 0.8990 | 0.8614 | 0.8798 | 0.8758
O | 40637 | 0.9688 | 0.9627 | 0.9657 | 0.9542
P | 2183 | 0.8512 | 0.6422 | 0.7321 | 0.7364
S | 2922 | 0.9878 | 0.9706 | 0.9791 | 0.9788
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BENCHMARK REPORT (Filtered Test Set)
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Total Residues Evaluated: 2500818
Overall Accuracy: 0.9838
Overall MCC: 0.9087
Balanced Accuracy: 0.8893
Macro Precision/Recall/F1:0.9133 / 0.8893 / 0.9005
Weighted P/R/F1: 0.9856 / 0.9838 / 0.9846
Micro P/R/F1: 0.9856 / 0.9838 / 0.9847
Classes absent in y_true for this benchmark split: P
Mean Sequence Accuracy: 0.9719
Median Sequence Accuracy: 1.0000
Sequence Exact Match: 0.8406
[Per-Protein Classification]
Protein Accuracy: 0.9806
Protein MCC: 0.9402
Protein Macro P/R/F1: 0.8568 / 0.9384 / 0.8799
Type | Support | Precision | Recall | F1
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GLOBULAR | 4044 | 0.9940 | 0.9879 | 0.9909
SIGNAL | 273 | 0.9464 | 0.9707 | 0.9584
TM | 566 | 0.9360 | 0.9558 | 0.9458
SP+TM | 15 | 0.5000 | 1.0000 | 0.6667
BETA | 63 | 0.9074 | 0.7778 | 0.8376
[Signal Peptide Metrics]
SP Presence Acc/MCC: 0.9950 / 0.9590
SP Presence P/R/F1: 0.9307 / 0.9936 / 0.9611
CS Exact Accuracy (true SP): 0.8167
CS Relaxed@±2 (true SP): 0.9068
CS Exact P/R/F1: 0.7651 / 0.8167 / 0.7900
CS Relaxed P/R/F1: 0.8494 / 0.9068 / 0.8771
[Per-Segment Metrics]
Segment | TP | FP | FN | Precision | Recall | F1 | BoundaryMAE
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B | 700 | 256 | 111 | 0.7322 | 0.8631 | 0.7923 | 1.3743
M | 2359 | 1725 | 668 | 0.5776 | 0.7793 | 0.6635 | 1.9532
S | 287 | 70 | 24 | 0.8039 | 0.9228 | 0.8593 | 0.1498
TM | 3059 | 1981 | 779 | 0.6069 | 0.7970 | 0.6891 | 1.8207
[Per-Class Performance (Residue Level)]
Class | Support | Precision | Recall | F1 | MCC | Specificity
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B | 7210 | 0.7967 | 0.7456 | 0.7703 | 0.7701 | 0.9994
I | 2263185 | 0.9945 | 0.9931 | 0.9938 | 0.9355 | 0.9480
M | 64393 | 0.9080 | 0.8096 | 0.8560 | 0.8539 | 0.9978
O | 158275 | 0.8990 | 0.9331 | 0.9157 | 0.9101 | 0.9929
P | 0 | 0.0000 | 0.0000 | 0.0000 | 0.0000 | 0.9981
S | 7755 | 0.9683 | 0.9649 | 0.9666 | 0.9665 | 0.9999
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