载体基本信息 | |||
载体名称 | pUCATPH | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5086 bp | 载体类型 | Yeast Plasmids |
载体来源 | Yang G, Turgeon BG, Yoder OC. | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pUCATPH 载体载体图谱
pUCATPH 载体载体序列
LOCUS Exported 5086 bp ds-DNA circular SYN 11-3-2013 DEFINITION Hygromycin resistance plasmid for restriction enzyme-mediated integration (REMI) mutagenesis of fungi. ACCESSION . VERSION . KEYWORDS pUCATPH SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5086) AUTHORS Yang G, Turgeon BG, Yoder OC. TITLE Toxin-deficient mutants from a toxin-sensitive transformant of Cochliobolus heterostrophus. JOURNAL Genetics 1994;137:751-7. PUBMED 8088521 REFERENCE 2 (bases 1 to 5086) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5086 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 379..395 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" terminator 704..1262 /note="trpC terminator" /note="terminator for Aspergillus nidulans trpC" CDS complement(1431..2456) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" gene complement(1431..2456) /gene="hph" /note="hph" promoter complement(2461..2817) /note="trpC promoter" /note="promoter for Aspergillus nidulans trpC" primer_bind complement(2865..2881) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 2889..2905 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2913..2943) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(3267..3855) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4026..4886) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4887..4991) /gene="bla" /note="AmpR promoter"
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