pUCATPH 载体 (V010214#) COA报告 测序报告

载体基本信息
载体名称 pUCATPH 载体抗性 Ampicillin
载体长度 5086 bp 载体类型 Yeast Plasmids
载体来源 Yang G, Turgeon BG, Yoder OC. 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

pUCATPH 载体载体图谱

pUCATPH5086 bp6001200180024003000360042004800M13 fwdtrpC terminatorHygRtrpC promoterM13 revlac operatorlac promoteroriAmpRAmpR promoter

pUCATPH 载体载体序列

LOCUS       Exported                5086 bp ds-DNA    circular SYN 11-3-2013
DEFINITION  Hygromycin resistance plasmid for restriction enzyme-mediated 
            integration (REMI) mutagenesis of fungi.
ACCESSION   .
VERSION     .
KEYWORDS    pUCATPH
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Yang G, Turgeon BG, Yoder OC.
  TITLE     Toxin-deficient mutants from a toxin-sensitive transformant of 
            Cochliobolus heterostrophus.
  JOURNAL   Genetics 1994;137:751-7.
  PUBMED    8088521
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      704..1262
                     /note="trpC terminator"
                     /note="terminator for Aspergillus nidulans trpC"
     CDS             complement(1431..2456)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            complement(1431..2456)
                     /gene="hph"
                     /note="hph"
     promoter        complement(2461..2817)
                     /note="trpC promoter"
                     /note="promoter for Aspergillus nidulans trpC"
     primer_bind     complement(2865..2881)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2889..2905
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2913..2943)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4026..4886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4887..4991)
                     /gene="bla"
                     /note="AmpR promoter"

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