载体基本信息 | |||
载体名称 | pFA6a-GFP(S65T)-kanMX6 | 载体抗性 | Ampicillin |
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载体长度 | 4854 bp | 载体类型 | Yeast Plasmids |
载体来源 | Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, | 拷贝数 | High copy number |
pFA6a-GFP(S65T)-kanMX6 载体载体图谱
pFA6a-GFP(S65T)-kanMX6 载体载体序列
LOCUS Exported 4854 bp ds-DNA circular SYN 24-12-2013 DEFINITION Plasmid with a kanMX marker for adding a C-terminal GFP tag or truncating a gene. ACCESSION . VERSION . KEYWORDS pFA6a-GFP(S65T)-kanMX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4854) AUTHORS Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. TITLE Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. JOURNAL Yeast 1998;14:953-61. PUBMED 9717241 REFERENCE 2 (bases 1 to 4854) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4854 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" CDS 64..776 /codon_start=3 /gene="gfp" /product="green fluorescent protein, S65T variant" /note="GFP(S65T)" /db_xref="GI:1019894" /protein_id="AAA79306.1" /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV TAAGITHGMDELYK" terminator 797..984 /note="ADH1 terminator" gene 1031..2387 /note="kanMX" /note="yeast selectable marker conferring kanamycin resistance" promoter 1031..1374 /note="TEF promoter" /note="Ashbya gossypii TEF promoter" CDS 1375..2184 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" terminator 2190..2387 /note="TEF terminator" /note="Ashbya gossypii TEF terminator" promoter complement(2492..2510) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2768..3356) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(3527..4387) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4388..4492) /gene="bla" /note="AmpR promoter" promoter 4838..2 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase"
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