载体基本信息 | |||
载体名称 | pFA6a-bleMX6 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3503 bp | 载体类型 | Yeast Plasmids |
载体来源 | Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM. | 拷贝数 | High copy number |
pFA6a-bleMX6 载体载体图谱
pFA6a-bleMX6 载体载体序列
LOCUS Exported 3503 bp ds-DNA circular SYN 24-12-2013 DEFINITION Plasmid for yeast gene deletion using the bleMX6 selectable marker conferring phleomycin resistance. ACCESSION . VERSION . KEYWORDS pFA6a-bleMX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3503) AUTHORS Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM. TITLE Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. JOURNAL Yeast 2005;22:1013-9. PUBMED 16200533 REFERENCE 2 (bases 1 to 3503) AUTHORS EUROSCARF TITLE Direct Submission FEATURES Location/Qualifiers source 1..3503 /organism="synthetic DNA construct" /lab_host="Schizosaccharomyces pombe" /mol_type="other DNA" gene 113..1034 /note="bleMX6" /note="yeast selectable marker conferring phleomycin resistance" promoter 113..456 /note="TEF promoter" /note="Ashbya gossypii TEF promoter" CDS 457..831 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /note="BleoR" /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" terminator 837..1034 /note="TEF terminator" /note="Ashbya gossypii TEF terminator" promoter complement(1139..1157) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(1415..2003) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2174..3034) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3035..3139) /gene="bla" /note="AmpR promoter" promoter 3485..3503 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase"
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