载体基本信息 | |||
载体名称 | pFA6a-5FLAG-kanMX6 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4312 bp | 载体类型 | Yeast Plasmids |
载体来源 | Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. | 拷贝数 | High copy number |
pFA6a-5FLAG-kanMX6 载体载体图谱
pFA6a-5FLAG-kanMX6 载体载体序列
LOCUS Exported 4312 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Tagging vector pFA6a-5FLAG-kanMX6, complete sequence. ACCESSION EU493337 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4312) AUTHORS Noguchi C, Garabedian MV, Malik M, Noguchi E. TITLE A vector system for genomic FLAG epitope-tagging in Schizosaccharomyces pombe JOURNAL Biotechnol J 3 (9-10), 1280-1285 (2008) PUBMED 18729046 REFERENCE 2 (bases 1 to 4312) AUTHORS Noguchi C, Garabedian M, Malik M, Noguchi E. TITLE Direct Submission JOURNAL Submitted (16-FEB-2008) Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N 15th Street, MS 497, Philadelphia, PA 19102, USA REFERENCE 3 (bases 1 to 4312) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4312 /organism="Tagging vector pFA6a-5FLAG-kanMX6" /mol_type="other DNA" /label=pFA6a derivative /note="pFA6a derivative" /db_xref="taxon:524039" CDS 66..248 /codon_start=1 /product="5 x FLAG" /label=5 x FLAG /protein_id="ACD03200.1" /translation="MDYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK HMGAPLLNKRISYDL" CDS 69..200 /codon_start=1 /product="five tandem FLAG(R) epitope tags" /label=5xFLAG /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK" misc_feature 215..443 /label=ADH1 terminator /note="ADH1 terminator" terminator 227..414 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" gene 489..1845 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 489..832 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 833..1642 /codon_start=1 /gene="kanR" /product="kanamycin and geneticin (G418) resistance protein" /label=kanR /protein_id="ACD03201.1" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene 833..1642 /gene="kanR" /label=kanR terminator 1648..1845 /label=TEF terminator /note="Ashbya gossypii TEF terminator" promoter complement(1950..1968) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2226..2814) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2985..3845) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=bla /protein_id="ACD03202.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(2985..3845) /gene="bla" /label=bla promoter complement(3846..3950) /gene="bla" /label=AmpR promoter promoter 4296..2 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
我们不提供该载体,载体信息仅供查询和参考。