载体基本信息 | |||
载体名称 | pFA6a-3HA-His3MX6 | 载体抗性 | Ampicillin |
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载体长度 | 4092 bp | 载体类型 | Yeast Plasmids |
载体来源 | Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, | 拷贝数 | High copy number |
pFA6a-3HA-His3MX6 载体载体图谱
pFA6a-3HA-His3MX6 载体载体序列
LOCUS Exported 4092 bp ds-DNA circular SYN 29-11-2013 DEFINITION Plasmid with a HIS3MX6 marker for adding a C-terminal triple-HA tag. ACCESSION . VERSION . KEYWORDS pFA6a-3HA-His3MX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4092) AUTHORS Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. TITLE Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. JOURNAL Yeast 1998;14:953-61. PUBMED 9717241 REFERENCE 2 (bases 1 to 4092) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4092 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" CDS 69..158 /codon_start=1 /product="three tandem HA epitope tags" /note="3xHA" /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA" terminator 191..378 /note="ADH1 terminator" gene 425..1625 /note="HIS3MX6" promoter 425..767 /note="TEF promoter" /note="Ashbya gossypii TEF promoter" CDS 769..1422 /codon_start=1 /gene="Schizosaccharomyces pombe his5" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /function=" " /EC_number=" " /note="S. pombe his5" /note="yeast auxotrophic marker; corresponds to S. cerevisiae HIS3" /experiment=" " /protein_id=" " /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL" terminator 1428..1625 /note="TEF terminator" /note="Ashbya gossypii TEF terminator" promoter complement(1730..1748) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2006..2594) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(2765..3625) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3626..3730) /gene="bla" /note="AmpR promoter" promoter 4076..2 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase"
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