载体基本信息 | |||
载体名称 | pFA6a-12Pk-kanMX6 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4789 bp | 载体类型 | Yeast Plasmids |
载体来源 | Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. | 拷贝数 | High copy number |
pFA6a-12Pk-kanMX6 载体载体图谱
pFA6a-12Pk-kanMX6 载体载体序列
LOCUS Exported 4789 bp ds-DNA circular SYN 24-12-2013 DEFINITION Plasmid with a kanMX marker for adding a C-terminal 12Pk tag. ACCESSION . VERSION . KEYWORDS pFA6a-12Pk-kanMX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4789) AUTHORS Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. TITLE New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. JOURNAL BioTechniques 2013;55:257-63. PUBMED 24215641 REFERENCE 2 (bases 1 to 4789) AUTHORS Noguchi Lab TITLE Direct Submission FEATURES Location/Qualifiers source 1..4789 /organism="synthetic DNA construct" /lab_host="Schizosaccharomyces pombe" /mol_type="other DNA" CDS 73..672 /codon_start=1 /product="12 tandem copies of the Pk (V5) epitope" /note="12Pk tag" /translation="GKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGL DSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGK PIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGL DSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDST" terminator 702..889 /gene="S. cerevisiae ADH1" /note="ADH1 terminator" /note="transcription terminator for alcohol dehydrogenase 1" gene 964..2320 /note="kanMX" /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 964..1307 /note="TEF promoter" /note="Ashbya gossypii TEF promoter" CDS 1308..2117 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" terminator 2123..2320 /note="TEF terminator" /note="Ashbya gossypii TEF terminator" promoter complement(2425..2443) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2701..3289) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3460..4320) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4321..4425) /gene="bla" /note="AmpR promoter" promoter 4771..4789 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase"
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