pESC-LEU is a yeast episomal vector with a LEU2 marker, for galactose-regulated expression and tagging of up to two genes.
| 载体基本信息 | |||
| 载体名称 | pESC-LEU | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 7758 bp | 载体类型 | Yeast Plasmids |
| 载体来源 | Agilent Technologies | 拷贝数 | High copy number |
pESC-LEU 载体载体图谱
pESC-LEU 载体载体序列
LOCUS 40924_17606 7758 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pESC-LEU, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7758)
AUTHORS Lu Q.
TITLE pESC-LEU
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7758)
AUTHORS Lu Q.
TITLE Direct Submission
JOURNAL Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 3 (bases 1 to 7758)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7758)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
Rd., La Jolla, CA 92037, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7758
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(666..1757)
/codon_start=1
/label=LEU2
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(1770..2174)
/label=LEU2 promoter
rep_origin complement(2474..2929)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
terminator complement(3012..3177)
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
CDS complement(3325..3348)
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
promoter 3395..4059
/label=GAL1,10 promoter
/note="divergent inducible promoter, regulated by Gal4"
promoter 4070..4088
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4106..4135
/codon_start=1
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
terminator 4165..4354
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin complement(4597..5185)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5359..6216)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6217..6321)
/label=AmpR promoter
rep_origin complement(6348..7690)
/direction=LEFT
/label=2u ori
/note="yeast 2u plasmid origin of replication"
我们不提供该载体，载体信息仅供查询和参考。