pAbAi 载体 (V010329#) COA报告 测序报告

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。

所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。

由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。

To use pAbAi in a one-hybrid assay, clone one or more copies of a cis-acting element into the MCS. To facilitate recombination of the vector with the host’s ura3-52 locus, linearize the vector with either BbsI or BstBI, then introduce the linearized vector into competent yeast cells to integrate into the chromsome via homologous recombination between the vector’s URA3 gene and the ura3-52 locus of the yeast strain.

载体名称:
pAbAi
载体抗性:
Ampicillin
载体长度:
4894 bp
载体类型:
Yeast Plasmids
复制子:
ori
宿主:
Yeast
载体来源:
Clontech
拷贝数:
High copy number
启动子:
URA3

pAbAi 载体载体图谱

pAbAi4894 bp6001200180024003000360042004800MCSAurRM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterURA3URA3 promoter

pAbAi 载体载体序列

LOCUS       pAbAi.        4894 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast integrating vector for cloning a DNA bait sequence to be used 
            in a one-hybrid screen.
ACCESSION   .
VERSION     .
KEYWORDS    pAbAi
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize with BstBI or BbsI to integrate at the URA3 locus.
FEATURES             Location/Qualifiers
     source          1..4894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..45
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             185..1387
                     /label=AurR
                     /note="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
     primer_bind     complement(1645..1661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1669..1685)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1693..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1738..1759)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2047..2635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2809..3666)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3667..3771)
                     /label=AmpR promoter
     CDS             complement(3878..4678)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4679..4894)
                     /label=URA3 promoter

我们不提供该载体,载体信息仅供查询和参考。