载体基本信息 | |||
载体名称 | pAbAi | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4894 bp | 载体类型 | Yeast Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
pAbAi 载体载体图谱
pAbAi 载体载体序列
LOCUS Exported 4894 bp ds-DNA circular SYN 08-2-2013 DEFINITION Yeast integrating vector for cloning a DNA bait sequence to be used in a one-hybrid screen. ACCESSION . VERSION . KEYWORDS pAbAi SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4894) AUTHORS Clontech TITLE Direct Submission COMMENT Linearize with BstBI or BbsI to integrate at the URA3 locus. FEATURES Location/Qualifiers source 1..4894 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" misc_feature 1..45 /note="MCS" /note="multiple cloning site" CDS 185..1390 /codon_start=1 /gene="S. cerevisiae AUR1 with a dominant F158Y mutation" /product="aureobasidin A-resistant mutant of inositol phosphorylceramide synthase" /note="AurR" /note="confers fungal resistance to Aureobasidin A" /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK RA" rep_origin complement(2047..2635) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2806..3666) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3667..3771) /gene="bla" /note="AmpR promoter" CDS complement(3875..4678) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /note="URA3" /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(4679..4894) /gene="S. cerevisiae URA3" /note="URA3 promoter"
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