载体基本信息 | |||
载体名称 | pGIPZ | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 11688 bp | 载体类型 | Viral Expression & Packaging Vectors |
载体来源 | Open Biosystems | 拷贝数 | High copy number |
pGIPZ 载体载体图谱
pGIPZ 载体载体序列
LOCUS Exported 11688 bp ds-DNA circular SYN 29-1-2014 DEFINITION Empty lentiviral vector for constitutive expression of an shRNAmir together with TurboGFP. ACCESSION . VERSION . KEYWORDS pGIPZ SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11688) AUTHORS Open Biosystems TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..11688 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" LTR 1..634 /note="5' LTR" /note="5' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2063..2180 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(2245..2619) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /note="BleoR" /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" promoter complement(2639..2686) /note="EM7 promoter" /note="synthetic bacterial promoter " enhancer 2791..3094 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 3095..3298 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 3390..4088 /codon_start=1 /product="improved green fluorescent protein from Pontellina plumata" /note="TurboGFP" /note="mammalian codon-optimized" /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF KTPDADAGEE" misc_feature 4115..4683 /note="IRES" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 4690..5292 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 5392..5650 /note="shRNAmir insertion site" /note="A fragment encoding a microRNA-adapted shRNA can be inserted between the XhoI and MluI sites." misc_feature 5663..6251 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 6458..6691 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6798..7022 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 7068..7496 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7510..7839 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 7690..7825 /note="SV40 ori" /note="SV40 origin of replication" CDS 7888..8913 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" polyA_signal 9043..9164 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(9615..10203) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10374..11234) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(11235..11339) /gene="bla" /note="AmpR promoter" polyA_signal 11387..11521 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal"
我们不提供该载体,载体信息仅供查询和参考。