pBABE-Puro 载体 (V010490#)

载体基本信息
载体名称 pBABE-Puro 载体抗性 Ampicillin
载体长度 5169 bp 载体类型 Viral Expression & Packaging Vectors
载体来源 Morgenstern JP, Land H. 筛选标记 Puromycin
拷贝数 High copy number 克隆方法 Restriction Enzyme
5'引物 pBABE 5' 3'引物 pBABE 3'

pBABE-Puro 载体载体图谱

pBABE-Puro5169 bp6001200180024003000360042004800LTR (truncated)MMLV Psigag (truncated)MCSSV40 promoterPuroRlong terminal repeat from Moloney murine leukemia virusoriAmpRAmpR promoter

pBABE-Puro 载体载体序列

LOCUS       Exported                5169 bp ds-DNA    circular SYN 29-11-2013
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
COMMENT     Strong expression is driven by the viral promoter in the 5' LTR.
            
            The commercially provided sequence was modified to include the 
            splice donor mutation.
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     LTR             8..479
                     /note="LTR (truncated)"
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    541
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /note="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /product="
                     "
                     /note="gag (truncated)"
                     /note="truncated MMLV gag gene lacking the start codon"
     misc_feature    1355..1402
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1593..1728
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             1752..2351
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     LTR             2450..3043
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(3415..4003)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4181..5041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5042..5146)
                     /gene="bla"
                     /note="AmpR promoter"

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