载体基本信息 | |||
载体名称 | pBI221 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5667 bp | 载体类型 | Plant Vectors |
载体来源 | Chen PY, Wang CK, Soong SC, To KY. | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pBI221 载体载体图谱
pBI221 载体载体序列
LOCUS 40924_6402 5667 bp DNA circular SYN 17-DEC-2018 DEFINITION Transient expression vector pBI221, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5667) AUTHORS Chen PY, Wang CK, Soong SC, To KY. TITLE Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants JOURNAL Mol. Breed. 11, 287-293 (2003) REFERENCE 2 (bases 1 to 5667) AUTHORS Chen PY, Wang CK, To KY. TITLE Direct Submission JOURNAL Submitted (12-APR-2002) Institute of Bioagricultural Sciences, Academia Sinica, Taipei 11529, Taiwan REFERENCE 3 (bases 1 to 5667) TITLE Direct Submission REFERENCE 4 (bases 1 to 5667) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Breed. 11, 287-293 (2003)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (12-APR-2002) Institute of Bioagricultural Sciences, Academia Sinica, Taipei 11529, Taiwan" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5667 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 512..857 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 896..2704 /codon_start=1 /label=GUS /note="beta-glucuronidase" /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK PKSAAFLLQKRWTGMNFGEKPQQGGKQ" terminator 2778..3030 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3039..3055) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3529..3633 /label=AmpR promoter CDS 3634..4491 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4665..5253 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5541..5562 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 5577..5607 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5615..5631 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5639..5655 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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