载体基本信息 | |||
载体名称 | pLacI | 载体抗性 | Chloramphenicol |
---|---|---|---|
载体长度 | 3813 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | Medium copy number |
pLacI 载体载体图谱
pLacI 载体载体序列
LOCUS Exported 3813 bp ds-DNA circular SYN 07-1-2013 DEFINITION lac repressor plasmid with a p15A origin of replication. ACCESSION . VERSION . KEYWORDS pLacI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3813) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission COMMENT pLacI can be propagated in E. coli cells that contain a second plasmid with the colE1 origin. FEATURES Location/Qualifiers source 1..3813 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter complement(220..322) /note="cat promoter" /note="promoter of the E. coli cat gene" rep_origin complement(848..1392) /direction=LEFT /note="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS complement(1617..2699) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(2700..2777) /gene="lacI" /note="lacI promoter" /note=" " CDS complement(3373..219) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
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