| 载体基本信息 | |||
| 载体名称 | pET-21a(+) | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 5443 bp | 载体类型 | pET & Duet Vectors (Novagen) |
| 载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-21a(+) 载体载体图谱
pET-21a(+) 载体载体序列
LOCUS Exported 5443 bp ds-DNA circular SYN 02-12-2013
DEFINITION Bacterial vector for inducible expression of N-terminally T7-tagged
proteins. For other reading frames, use pET-21b(+) or pET-21c(+).
ACCESSION .
VERSION .
KEYWORDS pET-21a(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5443)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
COMMENT
FEATURES Location/Qualifiers
source 1..5443
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 26..73
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
misc_feature 158..203
/note="MCS"
/note="multiple cloning site"
CDS complement(207..239)
/codon_start=1
/product="epitope tag from a T7 major capsid protein"
/note="T7 tag"
/translation="MASMTGGQQMG"
RBS 248..253
/note="ribosome binding site"
protein_bind 284..308
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(309..327)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 636..713
/gene="lacI"
/note="lacI promoter"
/note="
"
CDS 714..1796
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
CDS 2605..2796
/codon_start=1
/gene="rop"
/product="Rop protein"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
rep_origin complement(3226..3814)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3985..4845)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4846..4950)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(4977..5432)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
我们不提供该载体，载体信息仅供查询和参考。