载体基本信息 | |||
载体名称 | pEF1α-IRES | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6690 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Clontech | 拷贝数 | High copy number |
pEF1α-IRES 载体载体图谱
pEF1α-IRES 载体载体序列
LOCUS Exported 6690 bp ds-DNA circular SYN 26-1-2014 DEFINITION IRES-containing vector for expressing two genes in mammalian cells from the same bicistronic transcript. ACCESSION . VERSION . KEYWORDS pEF1alpha-IRES SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6690) AUTHORS Clontech TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..6690 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" promoter 154..1335 /note="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 384..1326 /note="EF-1-alpha intron A" /note="intron upstream of the start codon" intron 1475..1607 /note="chimeric intron" /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 1652..1670 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 1670..1692 /label="MCS A" /note="MCS A" /note="multiple cloning site" misc_feature 1737..2310 /note="IRES" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 2332..2355 /label="MCS B" /note="MCS B" /note="multiple cloning site" polyA_signal 2394..2515 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 2701..3156 /direction=RIGHT /label="f1 origin" /note="f1 origin" promoter 3273..3630 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 3481..3616 /label="SV40 origin" /note="SV40 origin" CDS 3681..4475 /codon_start=1 /label="Kan(R)/Neo(R)" /note="Kan" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter 4893..4997 /gene="bla" /note="AmpR promoter" CDS 4998..5858 /codon_start=1 /label="Amp(R)" /note="Amp" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6029..6617 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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