载体基本信息 | |||
载体名称 | pcDNA3.1/His C | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5513 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Invitrogen (Life Technologies) | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pcDNA3.1/His C 载体载体图谱
pcDNA3.1/His C 载体载体序列
LOCUS Exported 5513 bp ds-DNA circular SYN 28-11-2013 DEFINITION Mammalian vector for expressing proteins with a cleavable N-terminal 6xHis tag. For other reading frames, use pcDNA(TM)3.1/His A or pcDNA(TM)3.1/His B. ACCESSION . VERSION . KEYWORDS pcDNA3.1 His C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5513) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission FEATURES Location/Qualifiers source 1..5513 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 235..614 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 920..922 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 932..949 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS 989..1012 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /note="Xpress(TM) tag" /translation="DLYDDDDK" misc_feature 1012..1084 /note="MCS" /note="multiple cloning site" polyA_signal 1110..1334 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 1380..1808 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1822..2151 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2002..2137 /note="SV40 ori" /note="SV40 origin of replication" CDS 2218..3012 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3186..3307 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(3758..4346) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4517..5377) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5378..5482) /gene="bla" /note="AmpR promoter"
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