pBI-CMV1 载体 (V011351#) COA报告 测序报告

pBI-CMV1, this is a mammalian bidirectional promoter vector for constitutively expressing two genes at similar levels.

载体基本信息
载体名称 pBI-CMV1 载体抗性 Ampicillin
载体长度 3114 bp 载体类型 Mammalian Expression Vectors
载体来源 Clontech 拷贝数 High copy number

pBI-CMV1 载体载体图谱

pBI-CMV13114 bp6001200180024003000CMV enhancerCMV promoterMCS 1SV40 poly(A) signaloriAmpRAmpR promoterSV40 poly(A) signalMCS 2minimal CMV promoter

pBI-CMV1 载体载体序列

LOCUS       pBI-CMV1.        3114 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian bidirectional promoter vector for constitutively 
            expressing two genes at similar levels.
ACCESSION   .
VERSION     .
KEYWORDS    pBI-CMV1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The gene inserted into each MCS should contain start and stop 
            codons.
FEATURES             Location/Qualifiers
     source          1..3114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    602..663
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     polyA_signal    777..858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1040..1628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1802..2659)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2660..2764)
                     /label=AmpR promoter
     polyA_signal    complement(2797..2878)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2986..3040
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     promoter        complement(3069..3106)
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"

我们不提供该载体,载体信息仅供查询和参考。