载体基本信息 | |||
载体名称 | p3xFLAG-CMV-8 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4763 bp | 载体类型 | Mammalian Expression Vectors |
载体来源 | Sigma-Aldrich | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
p3xFLAG-CMV-8 载体载体图谱
p3xFLAG-CMV-8 载体载体序列
LOCUS Exported 4763 bp ds-DNA circular SYN 05-1-2013 DEFINITION Vector for expression and secretion of N-terminally 3xFLAG-tagged proteins in mammalian cells. ACCESSION . VERSION . KEYWORDS p3xFLAG-CMV-8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4763) AUTHORS Sigma-Aldrich TITLE Direct Submission FEATURES Location/Qualifiers source 1..4763 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" primer_bind 141..157 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" enhancer 318..697 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 698..901 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 932..976 /codon_start=1 /product="leader sequence from mouse preprotrypsin" /note="preprotrypsin leader" /translation="MSALLILALVGAAVA" CDS 977..1042 /codon_start=1 /product="three tandem FLAG epitope tags, followed by an enterokinase cleavage site" /note="3xFLAG" /translation="DYKDHDGDYKDHDIDYKDDDDK" misc_feature 1040..1106 /note="MCS" /note="multiple cloning site" polyA_signal 1104..1726 /note="hGH poly(A) signal" /note="human growth hormone polyadenylation signal" rep_origin 1935..2070 /note="SV40 ori" /note="SV40 origin of replication" primer_bind complement(2155..2171) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2557..3145) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3316..4176) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4177..4281) /gene="bla" /note="AmpR promoter" rep_origin 4308..4763 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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