| 载体基本信息 | |||
| 载体名称 | p3xFLAG-CMV-14 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 6310 bp | 载体类型 | Mammalian Expression Vectors |
| 载体来源 | Sigma-Aldrich | 筛选标记 | Neomycin/G418(Geneticin) |
| 拷贝数 | High copy number | 启动子 | CMV |
| 克隆方法 | Enzyme digestion and ligation | 5'引物 | M13 fwd |
| 3'引物 | M13 rev | ||
p3xFLAG-CMV-14 载体载体图谱
p3xFLAG-CMV-14 载体载体序列
LOCUS Exported 6310 bp ds-DNA circular SYN 28-11-2013
DEFINITION Vector with a neomycin resistance marker for expression of
C-terminally 3xFLAG-tagged proteins in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS p3xFLAG-CMV-14
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6310)
AUTHORS Sigma-Aldrich
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6310
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
primer_bind 141..157
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
enhancer 318..697
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 698..901
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 924..990
/note="MCS"
/note="multiple cloning site"
CDS 993..1058
/codon_start=1
/product="three tandem FLAG epitope tags, followed by an
enterokinase cleavage site"
/note="3xFLAG"
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 1069..1691
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
promoter 1733..2030
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2084..2878
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3530..3664
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(3702..3718)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(4104..4692)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4863..5723)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5724..5828)
/gene="bla"
/note="AmpR promoter"
rep_origin 5855..6310
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
我们不提供该载体，载体信息仅供查询和参考。