| 载体基本信息 | |||
| 载体名称 | pNL1.1.CMV[Nluc/CMV] | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 3861 bp | 载体类型 | Luciferase Vectors |
| 载体来源 | Promega | 拷贝数 | High copy number |
pNL1.1.CMV[Nluc/CMV] 载体载体图谱
pNL1.1.CMV[Nluc/CMV] 载体载体序列
LOCUS Exported 3861 bp ds-DNA circular SYN 26-7-2013
DEFINITION Co-transfection vector for expressing NanoLuc(R) luciferase under
control of the strong CMV promoter.
ACCESSION JQ513379
VERSION .
KEYWORDS pNL1.1.CMV[Nluc CMV]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3861)
AUTHORS Promega
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3861
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 227..530
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 531..734
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 859..1374
/codon_start=1
/gene="
"
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/protein_id="
"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
polyA_signal 1415..1536
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1955..2543)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2743..3603)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3708..3756
/note="synthetic polyadenylation signal"
misc_feature 3770..3861
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
我们不提供该载体，载体信息仅供查询和参考。