载体基本信息 | |||
载体名称 | pNL1.1.CMV[Nluc/CMV] | 载体抗性 | Ampicillin |
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载体长度 | 3861 bp | 载体类型 | Luciferase Vectors |
载体来源 | Promega | 拷贝数 | High copy number |
pNL1.1.CMV[Nluc/CMV] 载体载体图谱
pNL1.1.CMV[Nluc/CMV] 载体载体序列
LOCUS Exported 3861 bp ds-DNA circular SYN 26-7-2013 DEFINITION Co-transfection vector for expressing NanoLuc(R) luciferase under control of the strong CMV promoter. ACCESSION JQ513379 VERSION . KEYWORDS pNL1.1.CMV[Nluc CMV] SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3861) AUTHORS Promega TITLE Direct Submission FEATURES Location/Qualifiers source 1..3861 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 227..530 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 531..734 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 859..1374 /codon_start=1 /gene=" " /product="NanoLuc(R) luciferase" /note="Nluc" /note="human codon-optimized" /protein_id=" " /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILA" polyA_signal 1415..1536 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(1955..2543) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2743..3603) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 3708..3756 /note="synthetic polyadenylation signal" misc_feature 3770..3861 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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