下载GeneBank文件(.gb)

LOCUS       pGEM-luc.        4931 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid carrying the firefly luciferase gene flanked by unique 
            restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-luc.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Derived from pGEM(R)-11Zf(–).
FEATURES             Location/Qualifiers
     source          1..4931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..50
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(105..1754)
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    1757..1795
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1802..1820)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1838..1854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1862..1878)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1886..1916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1931..1952)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2240..2828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3002..3859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3860..3964)
                     /label=AmpR promoter
     rep_origin      4296..4751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4892..4908
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4915..4931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"