- 载体名称:
- pCMV-Gaussia-Dura Luc
- 载体抗性:
- Ampicillin
- 载体长度:
- 5482 bp
- 载体类型:
- Luciferase Vectors
- 复制子:
- ori
- 载体来源:
- Thermo Scientific (Pierce)
- 拷贝数:
- High copy number
- 启动子:
- EM7
pCMV-Gaussia-Dura Luc 载体载体图谱
pCMV-Gaussia-Dura Luc 载体载体序列
LOCUS 40924_11650 5482 bp DNA circular SYN 01-JAN-1980 DEFINITION Control vector for constitutive high-level expression of secreted Gaussia-Dura luciferase under control of the CMV promoter. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5482) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 5482) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5482 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 63..366 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 367..570 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 615..633 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 646..1200 /codon_start=1 /label=Gaussia-Dura Luc /note="enhanced variant of secreted Gaussia luciferase" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ RCATFASKIQGQVDKIKGAGGD" polyA_signal 1226..1337 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 1460..1789 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 1837..1884 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 1903..2499 /codon_start=1 /label=PuroR /note="puromycin N-acetyltransferase" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 2632..2765 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(2809..3666) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3930..4518 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4788..4804 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 5260..5276 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 5391..5482 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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