下载GeneBank文件(.gb)

LOCUS       pCLuc_Mini-TK_2.        6131 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector with a minimal promoter for measuring promoter or 
            enhancer activity using secreted Cypridina luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pCLuc Mini-TK 2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..68
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        69..131
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             145..1803
                     /label=CLuc
                     /note="secreted Cypridina luciferase"
     polyA_signal    1815..1863
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      1997..2425
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2439..2769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2836..3627
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3804..3937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3974..3990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3998..4014)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4022..4052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4067..4088)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4376..4964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5138..5995)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5996..6100)
                     /label=AmpR promoter