| 载体基本信息 | |||
| 载体名称 | pBIND-GR | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 7548 bp | 载体类型 | Luciferase Vectors |
| 载体来源 | Promega | 筛选标记 | Neomycin/G418(Geneticin) |
| 拷贝数 | High copy number | ||
pBIND-GR 载体载体图谱
pBIND-GR 载体载体序列
LOCUS Exported 7548 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pBIND-GR, complete sequence.
ACCESSION GQ229580
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7548)
AUTHORS Kopish K, Pagiuo A, TenHarmsel A.
TITLE GloResponse(TM) 9XGAL4UAS-luc2P HEK293 Cell Line Technical Bulletin,
TB552
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7548)
AUTHORS Kopish K, Pagiuo A, TenHarmsel A.
TITLE Direct Submission
JOURNAL Submitted (02-JUN-2009) Scientific Communications, Promega
Corporation, 5500 E. Cheryl Parkway, Madison, WI 53711, USA
REFERENCE 3 (bases 1 to 7548)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7548
/organism="Cloning vector pBIND-GR"
/mol_type="other DNA"
/db_xref="taxon:659259"
regulatory 1..742
/regulatory_class="promoter"
/note="CMV immediate-early enhancer/promoter region"
enhancer 138..517
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 518..721
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 857..989
/note="chimeric intron"
regulatory 1033..1052
/regulatory_class="promoter"
/note="T7 RNA polymerase promoter"
promoter 1033..1051
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1083..1520
/codon_start=1
/gene="Gal4"
/product="Gal4 DNA binding domain"
/label=Gal4
/note="Gal4 DBD"
/protein_id="ACT53147.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV"
gene 1083..1520
/gene="Gal4"
/label=Gal4
misc_feature 1521..1553
/label=IgA linker
/note="IgA linker"
CDS 1563..2399
/codon_start=1
/gene="GR-LBD"
/product="GR LBD"
/label=GR-LBD
/note="glucocorticoid receptor ligand binding domain"
/protein_id="ACT53148.1"
/translation="MQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVL
YAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFL
MAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEE
YLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKL
LDSMHEVVENLLNYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
gene 1563..2399
/gene="GR-LBD"
/label=GR-LBD
regulatory 2559..2780
/regulatory_class="polyA_signal_sequence"
/note="SV40 late polyadenylation signal"
polyA_signal 2568..2689
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
regulatory 2879..3297
/regulatory_class="promoter"
/note="SV40 enhancer/early promoter"
promoter 2934..3291
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3142..3277
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 3336..3345
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3342..5117
/codon_start=1
/gene="hRluc-neo"
/product="hRluc-neo fusion protein"
/label=hRluc-neo
/note="renilla luciferase-neomycin phosphotransferase
fusion protein"
/protein_id="ACT53149.1"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQTGGGSGGGGSGGGGSGGIEQDGLHAGSPA
AWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLAT
TGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATC
PFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGD
ACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAP
DSQRIAFYRLLDEFF"
gene 3342..5117
/gene="hRluc-neo"
/label=hRluc-neo
CDS 3342..4274
/codon_start=1
/product="Renilla luciferase"
/label=hRluc
/note="human codon-optimized"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
CDS 4326..5117
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 5181..5499
/regulatory_class="polyA_signal_sequence"
/note="synthetic polyadenylation signal"
polyA_signal 5181..5229
/note="synthetic polyadenylation signal"
promoter 5385..5489
/gene="bla"
/label=AmpR promoter
CDS 5490..6350
/codon_start=1
/gene="Amp(R)"
/product="beta-lactamase"
/label=Amp(R)
/note="ampicillin resistance protein"
/protein_id="ACT53146.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene 5490..6350
/gene="Amp(R)"
/label=Amp(R)
rep_origin 6505..6541
/label=ColE1-derived plasmid origin of replication
/note="ColE1-derived plasmid origin of replication"
rep_origin complement(6508..7096)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 7212..7497
/label=cer
/note="cer"
misc_feature 7212..7495
/label=cer region
/note="ColE1-derived recombination site that helps to
maintain plasmids as monomers"
我们不提供该载体，载体信息仅供查询和参考。