载体基本信息 | |||
载体名称 | pBIND-GR | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7548 bp | 载体类型 | Luciferase Vectors |
载体来源 | Promega | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pBIND-GR 载体载体图谱
pBIND-GR 载体载体序列
LOCUS Exported 7548 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBIND-GR, complete sequence. ACCESSION GQ229580 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7548) AUTHORS Kopish K, Pagiuo A, TenHarmsel A. TITLE GloResponse(TM) 9XGAL4UAS-luc2P HEK293 Cell Line Technical Bulletin, TB552 JOURNAL Unpublished REFERENCE 2 (bases 1 to 7548) AUTHORS Kopish K, Pagiuo A, TenHarmsel A. TITLE Direct Submission JOURNAL Submitted (02-JUN-2009) Scientific Communications, Promega Corporation, 5500 E. Cheryl Parkway, Madison, WI 53711, USA REFERENCE 3 (bases 1 to 7548) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7548 /organism="Cloning vector pBIND-GR" /mol_type="other DNA" /db_xref="taxon:659259" regulatory 1..742 /regulatory_class="promoter" /note="CMV immediate-early enhancer/promoter region" enhancer 138..517 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 518..721 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 857..989 /note="chimeric intron" regulatory 1033..1052 /regulatory_class="promoter" /note="T7 RNA polymerase promoter" promoter 1033..1051 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1083..1520 /codon_start=1 /gene="Gal4" /product="Gal4 DNA binding domain" /label=Gal4 /note="Gal4 DBD" /protein_id="ACT53147.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV" gene 1083..1520 /gene="Gal4" /label=Gal4 misc_feature 1521..1553 /label=IgA linker /note="IgA linker" CDS 1563..2399 /codon_start=1 /gene="GR-LBD" /product="GR LBD" /label=GR-LBD /note="glucocorticoid receptor ligand binding domain" /protein_id="ACT53148.1" /translation="MQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVL YAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFL MAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQVSYEE YLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKL LDSMHEVVENLLNYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK" gene 1563..2399 /gene="GR-LBD" /label=GR-LBD regulatory 2559..2780 /regulatory_class="polyA_signal_sequence" /note="SV40 late polyadenylation signal" polyA_signal 2568..2689 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory 2879..3297 /regulatory_class="promoter" /note="SV40 enhancer/early promoter" promoter 2934..3291 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3142..3277 /label=SV40 ori /note="SV40 origin of replication" regulatory 3336..3345 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3342..5117 /codon_start=1 /gene="hRluc-neo" /product="hRluc-neo fusion protein" /label=hRluc-neo /note="renilla luciferase-neomycin phosphotransferase fusion protein" /protein_id="ACT53149.1" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQTGGGSGGGGSGGGGSGGIEQDGLHAGSPA AWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLAT TGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATC PFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGD ACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAP DSQRIAFYRLLDEFF" gene 3342..5117 /gene="hRluc-neo" /label=hRluc-neo CDS 3342..4274 /codon_start=1 /product="Renilla luciferase" /label=hRluc /note="human codon-optimized" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" CDS 4326..5117 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 5181..5499 /regulatory_class="polyA_signal_sequence" /note="synthetic polyadenylation signal" polyA_signal 5181..5229 /note="synthetic polyadenylation signal" promoter 5385..5489 /gene="bla" /label=AmpR promoter CDS 5490..6350 /codon_start=1 /gene="Amp(R)" /product="beta-lactamase" /label=Amp(R) /note="ampicillin resistance protein" /protein_id="ACT53146.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene 5490..6350 /gene="Amp(R)" /label=Amp(R) rep_origin 6505..6541 /label=ColE1-derived plasmid origin of replication /note="ColE1-derived plasmid origin of replication" rep_origin complement(6508..7096) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 7212..7497 /label=cer /note="cer" misc_feature 7212..7495 /label=cer region /note="ColE1-derived recombination site that helps to maintain plasmids as monomers"
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