| 载体基本信息 | |||
| 载体名称 | pBluescript SK(-) | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 2958 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
| 载体来源 | Short JM, Fernandez JM, Sorge JA, Huse WD. | 拷贝数 | High copy number |
| 5'引物 | M13 fwd | 3'引物 | M13 rev |
pBluescript SK(-) 载体载体图谱
pBluescript SK(-) 载体载体序列
LOCUS Exported 2958 bp ds-DNA circular SYN 04-1-2013
DEFINITION Standard cloning vector (phagemid excised from lambda ZAP). The f1
(-) orientation allows rescue of antisense strand ssDNA.
ACCESSION X52324
VERSION .
KEYWORDS pBluescript SK(-)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2958)
AUTHORS Short JM, Fernandez JM, Sorge JA, Huse WD.
TITLE Lambda ZAP: a bacteriophage lambda expression vector with in vivo
excision properties
JOURNAL Nucleic Acids Res. 16 (15), 7583-7600 (1988)
PUBMED 2970625
REFERENCE 2 (bases 1 to 2958)
AUTHORS Stratagene
TITLE Direct Submission
COMMENT
FEATURES Location/Qualifiers
source 1..2958
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
rep_origin 7..462
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(454..816)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSSKLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWKL"
primer_bind 603..619
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 653..760
/note="MCS"
/note="pBluescript multiple cloning site"
primer_bind 670..686
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(720..736)
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(773..791)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(812..828)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 836..852
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(860..890)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(1214..1802)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1973..2833)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2834..2938)
/gene="bla"
/note="AmpR promoter"
我们不提供该载体，载体信息仅供查询和参考。