载体基本信息 | |||
载体名称 | pLSSmOrange-N1 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 4723 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV. | 筛选标记 | Neomycin/G418(Geneticin) |
拷贝数 | High copy number |
pLSSmOrange-N1 载体载体图谱
pLSSmOrange-N1 载体载体序列
LOCUS Exported 4723 bp ds-DNA circular SYN 04-1-2013 DEFINITION Vector for fusing LSSmOrange to the C-terminus of a partner protein. ACCESSION . VERSION . KEYWORDS pLSSmOrange-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4723) AUTHORS Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV. TITLE An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging. JOURNAL J. Am. Chem. Soc. 2012;134:7913-23. PUBMED 22486524 REFERENCE 2 (bases 1 to 4723) AUTHORS Verkhusha Lab TITLE Direct Submission FEATURES Location/Qualifiers source 1..4723 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 60..364 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 591..671 /note="MCS" /note="multiple cloning site of fluorescent protein plasmids" CDS 679..1389 /codon_start=1 /product="monomeric orange fluorescent protein with a large Stokes shift" /note="LSSmOrange" /note="mammalian codon-optimized" /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" polyA_signal 1511..1632 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(1639..2094) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2121..2225 /gene="bla" /note="AmpR promoter" promoter 2227..2584 /note="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 2619..3413 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 3645..3692 /note="HSV TK poly(A) signal" /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 4021..4609 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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