载体基本信息 | |||
载体名称 | pdKeima570-S1 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3348 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | MBL International | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pdKeima570-S1 载体载体图谱
pdKeima570-S1 载体载体序列
LOCUS Exported 3348 bp ds-DNA circular SYN 04-1-2013 DEFINITION Vector for expressing dKeima570 in bacteria. ACCESSION . VERSION . KEYWORDS pdKeima570-S1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3348) AUTHORS MBL International TITLE Direct Submission FEATURES Location/Qualifiers source 1..3348 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 96..200 /gene="bla" /note="AmpR promoter" CDS 201..1061 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1232..1820 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2144..2174 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2206..2222 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 2264..2932 /codon_start=1 /product="dimeric Keima570 fluorescent protein" /note="dKeima570" /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV TKGGPLPFAWDILSPLMCYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTV SNDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK LEGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG " primer_bind complement(2954..2970) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants"
我们不提供该载体,载体信息仅供查询和参考。