载体基本信息 | |||
载体名称 | pdKeima570-S1 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3348 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | MBL International | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pdKeima570-S1 载体载体图谱
pdKeima570-S1 载体载体序列
LOCUS 40924_14740 3348 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for expressing dKeima570 in bacteria. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3348) AUTHORS MBL International TITLE Direct Submission REFERENCE 2 (bases 1 to 3348) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3348 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 96..200 /label=AmpR promoter CDS 201..1058 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1232..1820 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2108..2129 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2206..2222 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 2264..2929 /codon_start=1 /label=dKeima570 /note="dimeric Keima570 fluorescent protein" /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV TKGGPLPFAWDILSPLMCYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTV SNDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK LEGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG " primer_bind complement(2954..2970) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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