pDG3-S1 载体 (V012051#)

载体基本信息
载体名称 pDG3-S1 载体抗性 Ampicillin
载体长度 3357 bp 载体类型 Fluorescent Protein Genes & Plasmids
载体来源 MBL International 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

pDG3-S1 载体载体图谱

pDG3-S13357 bp6001200180024003000AmpR promoterAmpRorilac promoterlac operatorM13 revDronpa-Green3M13 fwd

pDG3-S1 载体载体序列

LOCUS       Exported                3357 bp ds-DNA    circular SYN 04-1-2013
DEFINITION  Vector for expressing Dronpa-Green3 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pDG3-S1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   MBL International
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        96..200
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2144..2174
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2264..2941
                     /codon_start=1
                     /product="photoswitchable Dronpa-Green3 fluorescent 
                     protein"
                     /note="Dronpa-Green3"
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDINAALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     primer_bind     complement(2963..2979)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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