载体基本信息 | |||
载体名称 | pAcGFP1 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3354 bp | 载体类型 | Fluorescent Protein Genes & Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
3'引物 | M13 rev |
pAcGFP1 载体载体图谱
pAcGFP1 载体载体序列
LOCUS Exported 3354 bp ds-DNA circular SYN 04-1-2013 DEFINITION Vector for expressing AcGFP1 in bacteria. ACCESSION . VERSION . KEYWORDS pAcGFP1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3354) AUTHORS Clontech TITLE Direct Submission COMMENT Translation from the lacZ start codon produces a fusion protein in E. coli. FEATURES Location/Qualifiers source 1..3354 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 143..173 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 181..197 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 217..219 /codon_start=1 /product="lacZ start codon" /note="ATG" /translation="M" misc_feature 234..281 /label="5' MCS" /note="5' MCS" /note="multiple cloning site" CDS 289..1008 /codon_start=1 /product="Aequorea coerulescens GFP" /note="AcGFP1" /note="mammalian codon-optimized" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF GFVTAAAITHGMDELYK" misc_feature 1009..1108 /label="3' MCS" /note="3' MCS" /note="multiple cloning site" promoter 1449..1553 /gene="bla" /note="AmpR promoter" CDS 1554..2414 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2585..3173 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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