载体基本信息 | |||
载体名称 | pBS SK(+) | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2964 bp | 载体类型 | Cloning Vectors |
拷贝数 | High copy number | 5'引物 | M13 fwd |
3'引物 | M13 rev |
pBS SK(+) 载体载体图谱
pBS SK(+) 载体载体序列
LOCUS Exported 2964 bp ds-DNA circular SYN 01-7-2015 DEFINITION Phagemid cloning vector, also known as BlueScribe SK Plus or BlueSKp. The MCS is reversed relative to pBS KS(+). ACCESSION L08787 VERSION . KEYWORDS pBS SK(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2964) AUTHORS . TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..2964 /organism="synthetic construct" /mol_type="genomic DNA" /db_xref="taxon:32630" rep_origin complement(3..458) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(241..816) /codon_start=1 /gene="lacZ (fragment)" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG FPRQALNRGLPLGFRFSALRHLDPKKLD" primer_bind 603..619 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 653..760 /note="MCS" /note="pBluescript multiple cloning site" promoter complement(773..791) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(812..828) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 836..852 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(860..890) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(1214..1802) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1973..2833) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2834..2938) /gene="bla" /note="AmpR promoter"
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