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LOCUS       LITMUS29.        2820 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High copy-number E. coli vector with a multiple cloning site flanked
            by mutant T7 promoters. The MCS is reversed relative to LITMUS28.
ACCESSION   .
VERSION     .
KEYWORDS    LITMUS29.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Evans PD, Cook SN, Riggs PD, Noren CJ.
  TITLE     LITMUS: multipurpose cloning vectors with a novel system for 
            bidirectional in vitro transcription.
  JOURNAL   BioTechniques 1995;19:130-5.
  PUBMED    7669286
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1995"; volume: "19"; pages: "130-5"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..142
                     /label=AmpR promoter
     CDS             143..1000
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1665..2253
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2350..2380
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2412..2428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2449..2466
                     /gene="lacZalpha"
                     /label=lacZalpha promoter
                     /note="T7 promoter (mutant)"
                     /note="mutant version of the promoter for bacteriophage T7
                     RNA polymerase"
     misc_feature    2460..2605
                     /gene="lacZalpha"
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2600..2617)
                     /note="T7 promoter (mutant)"
                     /note="mutant version of the promoter for bacteriophage T7
                     RNA polymerase"
     primer_bind     complement(2621..2637)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"