| 载体基本信息 | |||
| 载体名称 | pLD02X | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 9609 bp | 载体类型 | Load vector |
| 载体来源 | Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R. | ||
pLD02X 载体载体图谱
pLD02X 载体载体序列
LOCUS Exported 9609 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Load vector pLD02X, complete sequence.
ACCESSION KM457487
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9609)
AUTHORS Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R.
TITLE A load driver device for engineering modularity in biological
networks
JOURNAL Nat. Biotechnol. 32 (12), 1268-1275 (2014)
PUBMED 25419739
REFERENCE 2 (bases 1 to 9609)
AUTHORS Mishra D, Rivera-Ortiz PM, Lin A, Del Vecchio D, Weiss R.
TITLE Direct Submission
JOURNAL Submitted (02-SEP-2014) Synthetic Biology Center, MIT, 500
Technology Square, NE47, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 9609)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9609
/organism="Load vector pLD02X"
/mol_type="other DNA"
/db_xref="taxon:1565121"
regulatory 34..223
/regulatory_class="terminator"
/note="CYC1 terminator"
terminator 34..223
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin 410..1077
rep_origin complement(466..1054)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1225..2085)
/codon_start=1
/gene="ampR"
/product="AmpR"
/label=ampR
/protein_id="AIU94968.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene complement(1225..2085)
/gene="ampR"
/label=ampR
promoter complement(2086..2190)
/gene="bla"
/label=AmpR promoter
rep_origin 2217..3559
/label=2u ori
/note="yeast 2u plasmid origin of replication"
rep_origin 2219..3374
protein_bind complement(3180..3227)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 3828..4043
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 4044..4847
/codon_start=1
/gene="URA3"
/product="URA3"
/label=URA3
/protein_id="AIU94970.1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
gene 4044..4847
/gene="URA3"
/label=URA3
rep_origin complement(4978..5433)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin complement(5110..5416)
/direction=LEFT
terminator 5512..5677
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
regulatory complement(5512..5676)
/regulatory_class="terminator"
misc_feature 5917..5938
/label=attB4
/note="attB4"
protein_bind 5918..5938
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
regulatory 5972..6704
/regulatory_class="promoter"
misc_feature 6388..6427
/label=SKN7 binding site
/note="SKN7 binding site"
misc_feature 6428..6467
/label=SKN7 binding site
/note="SKN7 binding site"
protein_bind 6716..6740
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 6716..6737
/label=attP1
/note="attP1"
primer_bind 6886..6902
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6907..6931)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature complement(6909..6930)
/label=attB2
/note="attB2"
regulatory 6936..7147
/regulatory_class="terminator"
terminator 6954..7141
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
misc_feature 7244..7265
/label=attB4
/note="attB4"
protein_bind 7245..7265
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
regulatory 7299..8031
/regulatory_class="promoter"
misc_feature 7715..7754
/label=SKN7 binding site
/note="SKN7 binding site"
misc_feature 7755..7794
/label=SKN7 binding site
/note="SKN7 binding site"
protein_bind 8043..8067
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 8043..8064
/label=attP1
/note="attP1"
primer_bind 8213..8229
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(8234..8258)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature complement(8236..8257)
/label=attB2
/note="attB2"
regulatory 8263..8524
/regulatory_class="terminator"
terminator 8273..8520
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
CDS 8681..9490
/codon_start=1
/gene="kanR"
/product="KanR"
/label=kanR
/protein_id="AIU94969.1"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
gene 8681..9490
/gene="kanR"
/label=kanR
我们不提供该载体，载体信息仅供查询和参考。