载体基本信息 | |||
载体名称 | pLD02X | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 9609 bp | 载体类型 | Load vector |
载体来源 | Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R. |
pLD02X 载体载体图谱
pLD02X 载体载体序列
LOCUS Exported 9609 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Load vector pLD02X, complete sequence. ACCESSION KM457487 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9609) AUTHORS Mishra D, Rivera PM, Lin A, Del Vecchio D, Weiss R. TITLE A load driver device for engineering modularity in biological networks JOURNAL Nat. Biotechnol. 32 (12), 1268-1275 (2014) PUBMED 25419739 REFERENCE 2 (bases 1 to 9609) AUTHORS Mishra D, Rivera-Ortiz PM, Lin A, Del Vecchio D, Weiss R. TITLE Direct Submission JOURNAL Submitted (02-SEP-2014) Synthetic Biology Center, MIT, 500 Technology Square, NE47, Cambridge, MA 02139, USA REFERENCE 3 (bases 1 to 9609) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9609 /organism="Load vector pLD02X" /mol_type="other DNA" /db_xref="taxon:1565121" regulatory 34..223 /regulatory_class="terminator" /note="CYC1 terminator" terminator 34..223 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin 410..1077 rep_origin complement(466..1054) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1225..2085) /codon_start=1 /gene="ampR" /product="AmpR" /label=ampR /protein_id="AIU94968.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(1225..2085) /gene="ampR" /label=ampR promoter complement(2086..2190) /gene="bla" /label=AmpR promoter rep_origin 2217..3559 /label=2u ori /note="yeast 2u plasmid origin of replication" rep_origin 2219..3374 protein_bind complement(3180..3227) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 3828..4043 /gene="S. cerevisiae URA3" /label=URA3 promoter CDS 4044..4847 /codon_start=1 /gene="URA3" /product="URA3" /label=URA3 /protein_id="AIU94970.1" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" gene 4044..4847 /gene="URA3" /label=URA3 rep_origin complement(4978..5433) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" rep_origin complement(5110..5416) /direction=LEFT terminator 5512..5677 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" regulatory complement(5512..5676) /regulatory_class="terminator" misc_feature 5917..5938 /label=attB4 /note="attB4" protein_bind 5918..5938 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" regulatory 5972..6704 /regulatory_class="promoter" misc_feature 6388..6427 /label=SKN7 binding site /note="SKN7 binding site" misc_feature 6428..6467 /label=SKN7 binding site /note="SKN7 binding site" protein_bind 6716..6740 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6716..6737 /label=attP1 /note="attP1" primer_bind 6886..6902 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6907..6931) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature complement(6909..6930) /label=attB2 /note="attB2" regulatory 6936..7147 /regulatory_class="terminator" terminator 6954..7141 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" misc_feature 7244..7265 /label=attB4 /note="attB4" protein_bind 7245..7265 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" regulatory 7299..8031 /regulatory_class="promoter" misc_feature 7715..7754 /label=SKN7 binding site /note="SKN7 binding site" misc_feature 7755..7794 /label=SKN7 binding site /note="SKN7 binding site" protein_bind 8043..8067 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 8043..8064 /label=attP1 /note="attP1" primer_bind 8213..8229 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind complement(8234..8258) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature complement(8236..8257) /label=attB2 /note="attB2" regulatory 8263..8524 /regulatory_class="terminator" terminator 8273..8520 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" CDS 8681..9490 /codon_start=1 /gene="kanR" /product="KanR" /label=kanR /protein_id="AIU94969.1" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene 8681..9490 /gene="kanR" /label=kanR
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