载体基本信息 | |||
载体名称 | pMC210 | 载体抗性 | Kanamycin |
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载体长度 | 7153 bp | 载体类型 | Mycobacteria-E.coli shuttle probe vector |
载体来源 | Fan XY, Ma H, Guo J, Li ZM, Cheng ZH, Guo SQ, Zhao GP. |
pMC210 载体载体图谱
pMC210 载体载体序列
LOCUS Exported 7153 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Mycobacteria-E.coli shuttle probe vector pMC210, complete sequence. ACCESSION EU682406 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7153) AUTHORS Fan XY, Ma H, Guo J, Li ZM, Cheng ZH, Guo SQ, Zhao GP. TITLE A novel differential expression system for gene modulation in Mycobacteria JOURNAL Plasmid 61 (1), 39-46 (2009) PUBMED 18835406 REFERENCE 2 (bases 1 to 7153) AUTHORS Fan X-Y., Li Z-M., Ma H, Guo J, Zhao G-P. TITLE Analysis of promoter elements of Mycobacterium tuberculosis furA gene in vivo and their application for recombinant BCG to induce enhanced Th1 type immune responses JOURNAL Unpublished REFERENCE 3 (bases 1 to 7153) AUTHORS Fan X-Y., Ma H, Guo J, Li Z-M., Zhao G-P. TITLE Direct Submission JOURNAL Submitted (28-APR-2008) Department of Microbiology, Fudan University, Shanghai 200433, China REFERENCE 4 (bases 1 to 7153) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7153 /organism="Mycobacteria-E.coli shuttle probe vector pMC210" /mol_type="other DNA" /db_xref="taxon:529759" CDS 120..935 /codon_start=1 /gene="aph" /product="aminoglycoside-3'-phosphotransferase" /label=aph /note="kanamycin resistance; APH1 region of Tn903" /protein_id="ACD68590.1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene 120..935 /gene="aph" /label=aph rep_origin 1222..2009 /label=Escherichia coli OriE region derived from pUC19 /note="Escherichia coli OriE region derived from pUC19" rep_origin 1267..1855 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 2014..3909 /label=mycobacterial OriM region derived from pAL5000 /note="mycobacterial OriM region derived from pAL5000" misc_feature 3928..3971 /label=multicloning site /note="multicloning site" primer_bind 3953..3969 /label=SK primer /note="common sequencing primer, one of multiple similar variants" CDS 3971..7018 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /note="lacks first eight amino acids" /protein_id="ACD68591.1" /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" gene 3971..7018 /gene="lacZ" /label=lacZ terminator 7071..7117 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" regulatory 7074..7117 /regulatory_class="terminator" /note="rrnBT1"
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