下载GeneBank文件(.gb)

LOCUS       40924_31200        9837 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMON100407, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil 
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, 
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean 
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
  PUBMED    22363475
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 9837)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2011) Yield "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..621
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5'UTR           629..717
                     /label=derived from P. hybrida DnaK
                     /note="derived from P. hybrida DnaK"
     3'UTR           796..1110
                     /label=derived from G. barbadense E6
                     /note="derived from G. barbadense E6"
     misc_feature    1181..1205
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(1634..2522)
                     /label=derived from E. coli aadA
                     /note="derived from E. coli aadA"
     CDS             complement(1692..2480)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3053..3641
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3827..3967)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4072..4260)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      5528..6241
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6407..6431
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      6732..7331
                     /label=derived from A. thaliana Act7
                     /note="derived from A. thaliana Act7"
                     /regulatory_class="promoter"
     5'UTR           7332..7969
                     /label=derived from A. thaliana Act7
                     /note="derived from A. thaliana Act7"
     CDS             7972..8199
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label=EPSPS CTP
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17831.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             8200..9564
                     /gene="aroA"
                     /label=aroA
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from 
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     terminator      9574..9826
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"