载体基本信息 | |||
载体名称 | pMON100407 | 载体抗性 | Spectinomycin/Streptomycin |
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载体长度 | 9837 bp | 载体类型 | Expression vector |
载体来源 | Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME. |
pMON100407 载体载体图谱
pMON100407 载体载体序列
LOCUS Exported 9837 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pMON100407, complete sequence. ACCESSION JN400385 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9837) AUTHORS Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME. TITLE Expression of the Arabidopsis thaliana BBX32 Gene in Soybean Increases Grain Yield JOURNAL PLoS ONE 7 (2), E30717 (2012) PUBMED 22363475 REFERENCE 2 (bases 1 to 9837) AUTHORS Preuss SB. TITLE Direct Submission JOURNAL Submitted (27-JUL-2011) Yield & Stress Traits, Monsanto Company, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA REFERENCE 3 (bases 1 to 9837) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9837 /organism="Expression vector pMON100407" /mol_type="other DNA" /db_xref="taxon:1089331" regulatory 6..618 /regulatory_class="promoter" /note="CaMV 35S" promoter 276..621 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" 5'UTR 629..717 /label=derived from P. hybrida DnaK /note="derived from P. hybrida DnaK" 3'UTR 796..1110 /label=derived from G. barbadense E6 /note="derived from G. barbadense E6" misc_feature complement(1141..1497) /label=derived from A. tumifaciens T-DNA right border /note="derived from A. tumifaciens T-DNA right border" misc_feature 1181..1205 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" misc_feature complement(1634..2522) /label=derived from E. coli aadA /note="derived from E. coli aadA" CDS complement(1692..2480) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3053..3641 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 3827..3967 /label=bom /note="basis of mobility region from pBR322" CDS complement(4069..4260) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" rep_origin 5549..6163 /label=oriV /note="origin of replication for the bacterial F plasmid" rep_origin 5640..6171 /label=oriV /note="incP origin of replication" misc_feature complement(6252..6693) /label=derived from A. tumifaciens T-DNA left border /note="derived from A. tumifaciens T-DNA left border" misc_feature 6407..6431 /label=LB T-DNA repeat /note="left border repeat from octopine Ach5 T-DNA" regulatory 6732..7331 /regulatory_class="promoter" /note="derived from A. thaliana Act7" 5'UTR 7332..7969 /label=derived from A. thaliana Act7 /note="derived from A. thaliana Act7" CDS 7972..8199 /codon_start=1 /product="EPSPS CTP" /label=EPSPS CTP /note="derived from A. thaliana ShkG" /protein_id="AEP17831.1" /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC" CDS 8200..9567 /codon_start=1 /product="CP4 EPSPS" /label=CP4 EPSPS /note="derived from A. tumifaciens aroA-CP4" /protein_id="AEP17832.1" /translation="MLHGASSRPATARKSSGLSGTVRIPGDKSISHRSFMFGGLASGET RITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCR LTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKSEDGDRLPVTLRGPKTPT PITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGFGANLTVETDADG VRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILTL QEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPSMIDEYPILAVAAAFAEG ATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRPDGKGLGNASGAAVA THLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA" 3'UTR 9574..9826 /label=derived from A. tumifaciens nos /note="derived from A. tumifaciens nos" terminator 9574..9826 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal"
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