载体基本信息 | |||
载体名称 | pUC-mRYCE(R) | 载体抗性 | Ampicillin |
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载体长度 | 3917 bp | 载体类型 | BiFC expression vector |
载体来源 | Offenborn JN, Waadt R, Kudla J. |
pUC-mRYCE(R) 载体载体图谱
pUC-mRYCE(R) 载体载体序列
LOCUS Exported 3917 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION BiFC expression vector pUC-mRYCE(R), complete sequence. ACCESSION KR857701 VERSION . KEYWORDS BiFC expression vector. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3917) AUTHORS Offenborn JN, Waadt R, Kudla J. TITLE Visualization and translocation of ternary Calcineurin-A/Calcineurin-B/Calmodulin-2 protein complexes by dual-color trimolecular fluorescence complementation JOURNAL New Phytol. (2015) In press PUBMED 25919910 REFERENCE 2 (bases 1 to 3917) AUTHORS Offenborn JN, Waadt R, Kudla J. TITLE Direct Submission JOURNAL Submitted (22-MAY-2015) University of Muenster, Institute for Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany REFERENCE 3 (bases 1 to 3917) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3917 /organism="BiFC expression vector pUC-mRYCE(R)" /mol_type="other DNA" /db_xref="taxon:1662175" promoter 96..200 /gene="bla" /label=AmpR promoter regulatory 131..159 /regulatory_class="promoter" /note="amp prom" CDS 201..1061 /codon_start=1 /product="AmpR" /label=AmpR /protein_id="AKJ78209.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1216..1835 /label=pBR322 origin /note="pBR322 origin" rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2108..2129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" regulatory 2144..2173 /regulatory_class="promoter" /note="lac prom" protein_bind 2182..2198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2206..2222 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 2241..3104 /label=p35S /note="p35S" promoter 2751..3096 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 3111..3371 /codon_start=1 /product="mRYCE(R)" /label=mRYCE /protein_id="AKJ78208.1" /translation="MGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIK LDITSHNEDYTIVEQYERAEGRHSTGGMDELYKYPYDVPDYA" misc_feature 3114..3344 /label=mCherry 160-236 /note="mCherry 160-236" misc_feature 3345..3371 /label=HA-Tag /note="HA-Tag" CDS 3345..3371 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 3372..3422 /label=MCS2 /note="MCS2" misc_feature 3432..3696 /label=NosT /note="NosT" terminator 3442..3694 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3703..3719) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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