载体基本信息 | |||
载体名称 | pUC-TTrepT | 载体长度 | 5056 bp |
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载体类型 | Cloning vector | 载体来源 | Vieira J, Messing J. |
pUC-TTrepT 载体载体图谱
pUC-TTrepT 载体载体序列
LOCUS Exported 5056 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUC-TTrepT DNA, complete sequence. ACCESSION AB669567 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5056) AUTHORS Vieira J, Messing J. TITLE The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers JOURNAL Gene 19 (3), 259-268 (1982) PUBMED 6295879 REFERENCE 2 (bases 1 to 5056) AUTHORS Fujita A, Misumi Y, Koyama Y. TITLE Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZalpha gene and kanamycin or hygromycin resistance marker JOURNAL Plasmid 67 (3), 272-275 (2012) PUBMED 22252135 REFERENCE 3 (bases 1 to 5056) AUTHORS Fujita A. TITLE Direct Submission JOURNAL Submitted (08-SEP-2011) Contact:Atsushi Fujita National Institute of Advanced Science and Technology, Biomedical Research Institute; 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan URL :http://www.aist.go.jp/ REFERENCE 4 (bases 1 to 5056) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5056 /organism="Cloning vector pUC-TTrepT" /mol_type="other DNA" /note="derivative of pUC13 containing a new MCS and T. thermophilus repA gene; constructed for the E. coli-T. thremophilus shuttle vector" /db_xref="taxon:1085938" protein_bind 106..127 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 142..172 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 180..196 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 204..220 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 216..533 /note="E. coli beta-galactosidase gene (LacZ) alpha peptide" CDS 216..533 /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLAAASTRDLRYPVPNSLAVVLQRRDWENPGVTQLNRLA AHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDA A" misc_feature 233..283 /note="polylinker HindIII-NotI-SalI-NruI-AflII-EcoRV-Acc65I-EcoRI" primer_bind complement(284..300) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 690..3062 /label=derived from pYK225 (derivative of pTT8) /note="derived from pYK225 (derivative of pTT8)" CDS complement(1197..2360) /codon_start=1 /gene="repA" /product="putative RepA protein" /label=repA /protein_id="BAL70402.1" /translation="MVLRAYAALRGLSPEALRAHLLAPPLRPERAREAFQRPYLAHFAQ TLPRYPYATDDPKEGVRIYKRENALKRVHVQVGHYPHAVLRLVVDVDLPWPQVEERIHA LPPSLVLVNPRSGHFHAWYELDPIPLTPPPGREGSLKGALALLAEVEALLEAYYGADPG YNGLLSRNPFLHPPEWTWGGGKRWSLRDLHRELRGLLPSGTRRRVDPGLASYGRNNALF DRLRAEAYAHVALFRGVPGGEEAFRAWVEQRAHALNQSLFRDHPKGPLDPREVHHTAKS VAKWTYRNYRGARVYPVSSTGRPDRSRLSPQARALIPPLQGQELQEAVREGGRRRGSRR RQEAEEKLTEALKRLQARGERVTARALAREAGVKPHTASKWLKRMRE" gene complement(1197..2360) /gene="repA" /label=repA promoter 3150..3254 /gene="bla" /label=AmpR promoter CDS 3255..4115 /codon_start=1 /gene="Amp-R" /product="beta-lactamase" /label=Amp-R /protein_id="BAL70403.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene 3255..4115 /gene="Amp-R" /label=Amp-R misc_feature 4276..4861 /label=origin of replication (ori) in E. coli /note="origin of replication (ori) in E. coli" rep_origin 4286..4874 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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