载体基本信息 | |||
载体名称 | pWUR768 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 10193 bp | 载体类型 | Regulator-reporter vector |
载体来源 | van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, Kengen SW. |
pWUR768 载体载体图谱
pWUR768 载体载体序列
LOCUS Exported 10193 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Regulator-reporter vector pWUR768, complete sequence. ACCESSION KX670546 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10193) AUTHORS van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, Kengen SW. TITLE A growth- and bioluminescence-based bioreporter for the in vivo detection of novel biocatalysts JOURNAL Microb Biotechnol (2017) In press PUBMED 28393499 REFERENCE 2 (bases 1 to 10193) AUTHORS van Rossum T, Muras A, Baur MJ, Creutzburg SC, van der Oost J, Kengen SW. TITLE Direct Submission JOURNAL Submitted (04-AUG-2016) Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, Wageningen 6708 WE, The Netherlands REFERENCE 3 (bases 1 to 10193) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10193 /organism="Regulator-reporter vector pWUR768" /mol_type="other DNA" /db_xref="taxon:1979214" regulatory 10..87 /regulatory_class="promoter" /note="PlacIq" promoter 10..87 /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." regulatory 95..100 /regulatory_class="ribosome_binding_site" /note="RBS" CDS 107..985 /codon_start=1 /gene="araC" /product="AraC" /label=araC /note="transcriptional activator for the ara regulon" /protein_id="ARH53390.1" /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" gene 107..985 /gene="araC" /label=araC CDS complement(992..1807) /codon_start=1 /gene="kan" /product="aminoglycoside 3'-phosphotransferase" /label=kan /note="KmR; kanamycin resistance protein" /protein_id="ARH53391.1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene complement(992..1807) /gene="kan" /label=kan regulatory complement(1814..1819) /regulatory_class="ribosome_binding_site" /note="RBS" regulatory complement(1828..2138) /regulatory_class="promoter" /note="PBAD-adapt" rep_origin 2281..2951 /label=ColE1 /note="ColE1" rep_origin complement(2289..2877) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" regulatory 2959..3269 /regulatory_class="promoter" /note="PBAD-adapt" regulatory 3276..3281 /regulatory_class="ribosome_binding_site" /note="RBS" CDS 3288..4730 /codon_start=1 /gene="luxC" /product="fatty acid reductase" /label=luxC /note="LuxC; formation of aldehyde substrate for luciferase" /protein_id="ARH53392.1" /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK DVAVEIEQTRFLEEDKFLVFVP" gene 3288..4730 /gene="luxC" /label=luxC CDS 4743..5666 /codon_start=1 /gene="luxD" /product="acyl transferase" /label=luxD /note="LuxD; formation of aldehyde substrate for luciferase" /protein_id="ARH53393.1" /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS FEHLTIATVNERRMRIEIENQAISLS" gene 4743..5666 /gene="luxD" /label=luxD CDS 5715..6797 /codon_start=1 /gene="luxA" /product="luciferase alpha subunit" /label=luxA /note="LuxA" /protein_id="ARH53394.1" /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM KLFQSDVMPFLKEKQRSLLY" gene 5715..6797 /gene="luxA" /label=luxA CDS 6812..7795 /codon_start=1 /gene="luxB" /product="luciferase beta subunit" /label=luxB /note="LuxB" /protein_id="ARH53395.1" /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT" gene 6812..7795 /gene="luxB" /label=luxB CDS 7974..9086 /codon_start=1 /gene="luxE" /product="acyl-protein synthetase" /label=luxE /note="LuxE; formation of aldehyde substrate for luciferase" /protein_id="ARH53396.1" /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK YPGVLVEILRRVNTRTQKGCALSLTEAFDS" gene 7974..9086 /gene="luxE" /label=luxE regulatory 9101..9205 /regulatory_class="terminator" /note="T1 terminator" terminator 9106..9192 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" regulatory complement(9218..9312) /regulatory_class="terminator" /note="t0 terminator" terminator 9218..9312 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(9333..9992) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=cat /note="CmR; chloramphenicol resistance protein" /protein_id="ARH53397.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" gene complement(9333..9992) /gene="cat" /label=cat promoter complement(9993..10095) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase"
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