载体基本信息 | |||
载体名称 | pXUN | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5632 bp | 载体类型 | Transient expression vector |
载体来源 | Chen S, Songkumarn P, Liu J, Wang GL. |
pXUN 载体载体图谱
pXUN 载体载体序列
LOCUS Exported 5632 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Transient expression vector pXUN, complete sequence. ACCESSION FJ905203 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5632) AUTHORS Chen S, Songkumarn P, Liu J, Wang GL. TITLE A versatile zero background T-vector system for gene cloning and functional genomics JOURNAL Plant Physiol. 150 (3), 1111-1121 (2009) PUBMED 19403729 REFERENCE 2 (bases 1 to 5632) AUTHORS Chen S, Songkumarn P, Liu J, Wang G-L. TITLE Direct Submission JOURNAL Submitted (09-APR-2009) Department of Plant Pathology, The Ohio State University, 201 Kottman Hall, 2021 Coffey Rd, Columbus, OH 43210, USA REFERENCE 3 (bases 1 to 5632) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5632 /organism="Transient expression vector pXUN" /mol_type="other DNA" /db_xref="taxon:643600" regulatory complement(9..261) /regulatory_class="terminator" /note="Nos terminator" terminator 9..261 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS 622..927 /codon_start=1 /gene="ccdB" /product="CcdB" /label=ccdB /protein_id="ACR08728.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" gene 622..927 /gene="ccdB" /label=ccdB regulatory complement(995..2985) /regulatory_class="promoter" /note="maize ubiquitin-1 promoter" promoter complement(995..2985) /label=Ubi promoter /note="maize polyubiquitin gene promoter" primer_bind complement(3016..3032) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 3040..3056 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3064..3094) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3109..3130 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3386..4029) /direction=LEFT /label=derived from puc19 /note="derived from puc19" rep_origin complement(3418..4006) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4177..5037) /codon_start=1 /product="beta-lactamase" /label=beta-lactamase /note="confers resistance to ampicillin" /protein_id="ACR08729.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5038..5142) /gene="bla" /label=AmpR promoter primer_bind 5616..5632 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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