载体基本信息 | |||
载体名称 | pCVD005 | 载体抗性 | Kanamycin |
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载体长度 | 3315 bp | 载体类型 | Broad host range vector |
载体来源 | Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. |
pCVD005 载体载体图谱
pCVD005 载体载体序列
LOCUS Exported 3315 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Broad host range vector vector pCVD005, complete sequence. ACCESSION KM017940 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3315) AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. TITLE Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria JOURNAL Nucleic Acids Res. (2014) In press PUBMED 25074377 REFERENCE 2 (bases 1 to 3315) AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW. TITLE Direct Submission JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA REFERENCE 3 (bases 1 to 3315) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3315 /organism="Broad host range vector vector pCVD005" /mol_type="other DNA" /note="bla_S7942 is a device that confers ampicillin (Ap) resistance" /db_xref="taxon:1531863" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" misc_feature 370..375 /label=EcoRV /note="EcoRV" misc_feature complement(373..1403) /note="CVD-ANTR; device for the GC-adaptor assembly: antibiotic resistance markers" misc_feature complement(373..393) /note="C2G; GC-adaptor for seamless assembly, flanking antibiotic resistance markers, functional devices or custom sequences; synthetic" misc_feature 394..399 /label=AgeI /note="AgeI" regulatory 406..440 /regulatory_class="promoter" /note="PconII; -35 to +1 region derived from conII synthetic promoter; synthetic" promoter 406..434 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" regulatory 447..471 /regulatory_class="promoter" /note="luxA 5-UTR; ribosomal binding site" CDS 472..1332 /codon_start=1 /product="bla_S7942" /label=bla_S7942 /note="confers ampicillin resistance; codon optimized for Synechococcus elongatus PCC7942; amino acid sequence derived from pUC19 backbone; Synechococcus elongatus PCC7942" /protein_id="AII99787.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" regulatory 1267..1276 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 1341..1367 /regulatory_class="terminator" /note="trpA T; trpA terminator from Lorist6 INSD accession X98450, one nt shorter than reference; Lorist6" misc_feature 1377..1382 /label=NheI /note="NheI" misc_feature complement(1383..1403) /note="GC; GC-adaptor for seamless assembly, flanking antibiotic resistance markers or Escherichia coli origins to be assembled with a cyanobacterial replicon; synthetic" misc_feature 1401..1406 /label=EcoRV /note="EcoRV" rep_origin complement(1572..2245) /direction=LEFT /note="colE1_pMA-RQ; origin of replication of colE1 found inderived from pMA-RQ GeneART vector" rep_origin complement(1631..2219) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2393..3187) /codon_start=1 /product="KanR" /label=KanR /note="confers kanamycin resistance; derived from vector backbone pMK-RQ GeneART; GeneArt; Life Technologies" /protein_id="AII99786.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 2966..2971 /label=AgeI /note="AgeI" promoter complement(3188..3292) /gene="bla" /label=AmpR promoter regulatory complement(3188..3257) /regulatory_class="promoter" /note="Pbla; upstream region of the bla ORF including promoter and RBS; derived from pUC19"
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