载体基本信息 | |||
载体名称 | pYES2-EGFP | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6548 bp | 载体类型 | Yeast Plasmids |
筛选标记 | URA3 | 拷贝数 | High copy number |
启动子 | GAL1 promoter | 克隆方法 | Enzyme Cut |
5'引物 | EGFP-N-F: TGTACGGTGGGAGGTCTAT | 3'引物 | CYC1 Terminator: GTGACATAACTAATTACATGATG |
表达方法 | galactose induced |
pYES2-EGFP 载体载体图谱
pYES2-EGFP 载体载体序列
LOCUS Exported 6548 bp ds-DNA circular SYN 11-SEP-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pYES2-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6548) TITLE Direct Submission FEATURES Location/Qualifiers source 1..6548 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 4..251 /gene="S. cerevisiae CYC1" /note="CYC1 terminator" /note="transcription terminator for CYC1" promoter complement(1059..1077) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(1109..1551) /gene="S. cerevisiae GAL1" /note="GAL1 promoter" /note="inducible promoter, regulated by Gal4" protein_bind 1434..1551 /bound_moiety="Gal4" /note="UAS" /note="upstream activating sequence mediating Gal4-dependent induction" rep_origin 1570..2025 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" rep_origin 2094..2974 /note="2u ori" /note="yeast 2u plasmid origin of replication" promoter 3578..3793 /gene="S. cerevisiae URA3" /note="URA3 promoter" CDS 3794..4597 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /note="URA3" /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" CDS 4693..5553 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5724..6312 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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