| 载体基本信息 | |||
| 载体名称 | pYES2-EGFP | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 6548 bp | 载体类型 | Yeast Plasmids |
| 筛选标记 | URA3 | 拷贝数 | High copy number |
| 启动子 | GAL1 promoter | 克隆方法 | Enzyme Cut |
| 5'引物 | EGFP-N-F: TGTACGGTGGGAGGTCTAT | 3'引物 | CYC1 Terminator: GTGACATAACTAATTACATGATG |
| 表达方法 | galactose induced | ||
pYES2-EGFP 载体载体图谱
pYES2-EGFP 载体载体序列
LOCUS Exported 6548 bp ds-DNA circular SYN 11-SEP-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pYES2-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6548)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6548
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 4..251
/gene="S. cerevisiae CYC1"
/note="CYC1 terminator"
/note="transcription terminator for CYC1"
promoter complement(1059..1077)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(1109..1551)
/gene="S. cerevisiae GAL1"
/note="GAL1 promoter"
/note="inducible promoter, regulated by Gal4"
protein_bind 1434..1551
/bound_moiety="Gal4"
/note="UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
rep_origin 1570..2025
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 2094..2974
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
promoter 3578..3793
/gene="S. cerevisiae URA3"
/note="URA3 promoter"
CDS 3794..4597
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/note="URA3"
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
CDS 4693..5553
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5724..6312
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
我们不提供该载体，载体信息仅供查询和参考。