| 载体基本信息 | |||
| 载体名称 | pWPXLd | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 10455 bp | 载体类型 | Mammalian Expression, Lentiviral |
| 拷贝数 | High Copy | 克隆方法 | Restriction Enzyme |
| 5'引物 | See map | ||
pWPXLd 载体载体图谱
pWPXLd 载体载体序列
LOCUS Exported 10455 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pWPXLd
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10455)
TITLE Trono Lab Constitutive Lentiviral Plasmids
REFERENCE 2 (bases 1 to 10455)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10455
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 2..635
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 682..807
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1300..1533
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1718..1762
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 2120..3297
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2351..3288
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3245..3265
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
misc_feature 3345..3462
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
regulatory 3538..3547
/regulatory_class="other"
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3544..4260
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 3544..4260
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3589..3610)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3850..3869)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4197..4218
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4340..4928
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4393..4413)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4811..4822)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5017..5250
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(5275..5293)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5276..5293)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5583..5602)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(5816..5835)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5935..5957
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5995..6013)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6081..6185
/gene="bla"
/label=AmpR promoter
CDS 6186..7046
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6404..6423)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7217..7805
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7706..7725
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7959..7976
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(8046..8066)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8051..8380
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8231..8366
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8293..8312
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 9514..9579
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9709..9729
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 10154..10288
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10191..10210)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10245..10264
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
我们不提供该载体，载体信息仅供查询和参考。