pBSc393K 载体 (V009039#)

载体基本信息
载体名称 pBSc393K 载体抗性 Chloramphenicol
载体长度 4412 bp 载体类型 Cloning vector
载体来源 Radeck J, Meyer D, Lautenschlager N, Mascher T.

pBSc393K 载体载体图谱

pBSc393K4412 bp600120018002400300036004200CAP binding sitelac promoterlac operatorM13 revlambda t0 terminatorcat promoterCatincP origin of transferoribomRopT7Te terminatorAph3'rrnB T1 terminator

pBSc393K 载体载体序列

LOCUS       Exported                4412 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSc393K, complete sequence.
ACCESSION   KY995199
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Cloning vector pBSc393K"
                     /mol_type="other DNA"
                     /db_xref="taxon:2051880"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            235..558
                     /gene="lacZa*_18"
                     /label=lacZa*_18
     CDS             235..438
                     /codon_start=1
                     /gene="lacZa*_18"
                     /product="LacZa*_18"
                     /label=lacZa*_18
                     /protein_id="ATU32930.1"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPS"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        793..895
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             896..1555
                     /codon_start=1
                     /gene="cat"
                     /product="Cat"
                     /label=cat
                     /protein_id="ATU32931.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            896..1555
                     /gene="cat"
                     /label=cat
                     /note="cat (for E.coli)"
     oriT            1715..1823
                     /note="incP origin of transfer"
     rep_origin      1858..2446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2632..2772
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2874..3065)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop"
                     /label=rop
                     /protein_id="ATU32932.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            complement(2874..3065)
                     /gene="rop"
                     /label=rop
     terminator      3347..3374
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3386..4180)
                     /codon_start=1
                     /gene="aph3'"
                     /product="Aph3'"
                     /label=aph3'
                     /protein_id="ATU32933.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(3386..4180)
                     /gene="aph3'"
                     /label=aph3'
                     /note="aph3' (kan-res)"
     terminator      4320..4406
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"

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