载体基本信息 | |||
载体名称 | pBSc393K | 载体抗性 | Chloramphenicol |
---|---|---|---|
载体长度 | 4412 bp | 载体类型 | Cloning vector |
载体来源 | Radeck J, Meyer D, Lautenschlager N, Mascher T. |
pBSc393K 载体载体图谱
pBSc393K 载体载体序列
LOCUS Exported 4412 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBSc393K, complete sequence. ACCESSION KY995199 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4412) AUTHORS Radeck J, Meyer D, Lautenschlager N, Mascher T. TITLE Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis JOURNAL Sci Rep 7 (1), 14134 (2017) PUBMED 29074996 REFERENCE 2 (bases 1 to 4412) AUTHORS Radeck J. TITLE Direct Submission JOURNAL Submitted (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher Weg 20b, Dresden 01627, Germany REFERENCE 3 (bases 1 to 4412) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4412 /organism="Cloning vector pBSc393K" /mol_type="other DNA" /db_xref="taxon:2051880" protein_bind 125..146 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 161..191 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 199..215 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 223..239 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" gene 235..558 /gene="lacZa*_18" /label=lacZa*_18 CDS 235..438 /codon_start=1 /gene="lacZa*_18" /product="LacZa*_18" /label=lacZa*_18 /protein_id="ATU32930.1" /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPS" misc_feature 249..305 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(309..325) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" terminator 604..698 /label=lambda t0 terminator /note="transcription terminator from phage lambda" promoter 793..895 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 896..1555 /codon_start=1 /gene="cat" /product="Cat" /label=cat /protein_id="ATU32931.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" gene 896..1555 /gene="cat" /label=cat /note="cat (for E.coli)" oriT 1715..1823 /note="incP origin of transfer" rep_origin 1858..2446 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 2632..2772 /label=bom /note="basis of mobility region from pBR322" CDS complement(2874..3065) /codon_start=1 /gene="rop" /product="Rop" /label=rop /protein_id="ATU32932.1" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" gene complement(2874..3065) /gene="rop" /label=rop terminator 3347..3374 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(3386..4180) /codon_start=1 /gene="aph3'" /product="Aph3'" /label=aph3' /protein_id="ATU32933.1" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" gene complement(3386..4180) /gene="aph3'" /label=aph3' /note="aph3' (kan-res)" terminator 4320..4406 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene"
我们不提供该载体,载体信息仅供查询和参考。