载体基本信息 | |||
载体名称 | pCAGGS-pIL-1B | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6245 bp | 载体类型 | Mammalian expression vector |
载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pCAGGS-pIL-1B 载体载体图谱
pCAGGS-pIL-1B 载体载体序列
LOCUS Exported 6245 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Mammalian expression vector pCAGGS-pIL-1B, complete sequence. ACCESSION LT727210 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6245) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 6245) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 6245) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6245 /organism="Mammalian expression vector pCAGGS-pIL-1B" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4441." /db_xref="taxon:1944892" CDS 26..835 /codon_start=1 /note="unnamed protein product; hIL1B" /protein_id="SJL87765.1" /translation="MAEVPELASEMMAYYSGNEDDLFFEADGPKQMKCSFQDLDLCPLD GGIQLRISDHHYSKGFRQAASVVVAMDKLRKMLVPCPQTFQENDLSTFFPFIFEEEPIF FDTWDNEAYVHDAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFV QGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEI NNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS" misc_feature 836..1436 /label=3' UTR of hIL1B /note="3' UTR of hIL1B" gap 1437..1453 /estimated_length=17 regulatory 1535..1626 /regulatory_class="terminator" /note="3' UTR of rabbit beta-globin, incl. polyA" polyA_site 1602..1657 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" misc_feature 1627..1983 /label=3' flanking region of rabbit beta-globin /note="3' flanking region of rabbit beta-globin" primer_bind complement(2018..2034) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 2042..2058 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." regulatory complement(2061..2143) /regulatory_class="promoter" /note="lac promoter" promoter complement(2066..2096) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2131 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2190..2385 /label=SV40 promoter /note="SV40 early promoter" rep_origin 2236..2371 /label=SV40 ori /note="SV40 origin of replication" rep_origin 2285..2360 /label=SV40 ori /note="SV40 ori" polyA_signal 2391..2525 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" regulatory complement(2391..2465) /regulatory_class="terminator" /note="SV40 polyA late" regulatory 2391..2465 /regulatory_class="terminator" /note="SV40 polyA early" rep_origin complement(2580..2981) /direction=LEFT /label=pMB1 ori /note="pMB1 ori" rep_origin complement(2763..3351) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3522..4382) /codon_start=1 /note="unnamed protein product; ampicillin resistance" /protein_id="SJL87766.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4383..4487) /gene="bla" /label=AmpR promoter enhancer 4518..4897 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" regulatory 4518..4895 /regulatory_class="promoter" /note="hCMV-IE enhancer" promoter 4899..5176 /label=chicken beta-actin promoter regulatory 4901..6242 /regulatory_class="promoter" /note="AG promoter" intron 5177..6194 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" misc_feature 5270..6194 /label=intron AG promoter /note="intron AG promoter"
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