载体基本信息 | |||
载体名称 | pCBcth4 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5905 bp | 载体类型 | Cloning vector |
载体来源 | Zheng T, Cui J, Bae HR, Lynd LR, Olson DG. |
pCBcth4 载体载体图谱
pCBcth4 载体载体序列
LOCUS Exported 5905 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pCBcth4, complete sequence. ACCESSION MG026513 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5905) AUTHORS Zheng T, Cui J, Bae HR, Lynd LR, Olson DG. TITLE Expression of adhA from different organisms in Clostridium thermocellum JOURNAL Biotechnol Biofuels 10, 251 (2017) PUBMED 29213311 REFERENCE 2 (bases 1 to 5905) AUTHORS Zheng T. TITLE Direct Submission JOURNAL Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755, USA REFERENCE 3 (bases 1 to 5905) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5905 /organism="Cloning vector pCBcth4" /mol_type="other DNA" /db_xref="taxon:2059361" regulatory 1..209 /regulatory_class="promoter" /note="2638" CDS 210..1430 /codon_start=1 /gene="adhA" /product="AdhA" /label=adhA /note="derived from Thermococcus sp. strain AN1" /protein_id="AUE21777.1" /translation="MLWESQIPINQIFELRCRTIDYFGVGAINKFYDIAKDLKENRGIS RVILVTGKSSYKKCGAWDVVKPALEENGIEYVHYDKVGPNPTVDMVDEAAQMGKEFGAQ AVIGIGGGSPIDTAKSVAILLEYTDKTARELYKLQFSPRKAKPIIAINTTHGTGTEVNR FAVASILEEEYKPAIAYDCIYPLYSIDDPTLMTKLPADQTRYVTIDALNHVNEAATTKV ASPYSILLAKETARLIFDYLPEALIHPDNLQAKYYLLYASAIAGISFDNGLLHFTHALE HPLSAVKPDLPHGLGLAILLPAVIKHIYPATARILAEIYRPLVPEVKGVPGEAELVAKK VEEWLFAIGITQKLTDVGFSEDDVKRLTELAMTTPSLNLLLSLAPVEATKETIEAIYRD SLYPLSK" gene 210..1430 /gene="adhA" /label=adhA CDS complement(2288..3148) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3149..3240) /gene="bla" /label=AmpR promoter rep_origin 3840..4385 /direction=RIGHT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS 4856..5860 /codon_start=1 /gene="repB" /product="RepB replication protein" /label=repB /note="from Enterococcus faecalis plasmid pAM-alpha-1" /db_xref="GI:22652809" /protein_id="AAN03827.1" /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
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