载体基本信息 | |||
载体名称 | pAS2hA20R562A | 载体抗性 | Ampicillin |
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载体长度 | 10805 bp | 载体类型 | Yeast expression vector |
载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pAS2hA20R562A 载体载体图谱
pAS2hA20R562A 载体载体序列
LOCUS Exported 10805 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Yeast expression vector pAS2hA20R562A, complete sequence. ACCESSION LT727381 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10805) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 10805) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 10805) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10805 /organism="Yeast expression vector pAS2hA20R562A" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3771." /db_xref="taxon:1945452" rep_origin 2..1344 /label=2u ori /note="yeast 2u plasmid origin of replication" rep_origin complement(2..982) /direction=LEFT /label=2mu ori /note="2mu ori" protein_bind complement(965..1012) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 1603..1883 /gene="S. cerevisiae TRP1" /label=TRP1 promoter CDS 1884..2558 /codon_start=1 /note="unnamed protein product; TRP1" /protein_id="SJL88297.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin complement(2661..3116) /direction=LEFT /label=f1 ori /note="f1 ori" primer_bind 3261..3277 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" terminator 3314..3561 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" CDS complement(3855..4304) /codon_start=1 /note="unnamed protein product; CYH2" /protein_id="SJL88298.1" /translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRINM DKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVIDT LAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA" promoter 5072..5473 /gene="S. cerevisiae ADH1" /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" regulatory 5075..5478 /regulatory_class="promoter" /note="ADH1" CDS 5501..5941 /codon_start=1 /note="unnamed protein product; GALbd" /protein_id="SJL88299.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_feature 5957..5983 /label=HA-epitope /note="HA-epitope" CDS 5957..5983 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 5999..8371 /codon_start=1 /note="unnamed protein product; hA20m" /protein_id="SJL88300.1" /translation="MAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMHR YTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGNCL MHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRN WNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSLESGS NFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLVNRDRG RFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEANLPKEIN LVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNCPFFMSVN TQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNPEESTGGPH SAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASHAPDHTRHLD PGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQASKSDPSRLVRSPSP HSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFCTLCFIEYRENK HFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEAKRTEE QLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHPNQRMGPGAHRGEPA PEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG" terminator 8443..8630 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" regulatory 8443..8544 /regulatory_class="terminator" /note="ADH1T" primer_bind complement(8654..8670) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8678..8694 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8702..8732) /label=lac promoter /note="promoter for the E. coli lac operon" rep_origin complement(8872..9274) /direction=LEFT /label=pMB1 ori /note="pMB1 ori" rep_origin complement(9056..9644) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9815..10675) /codon_start=1 /note="unnamed protein product; ampicillin resistance" /protein_id="SJL88301.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10676..10780) /gene="bla" /label=AmpR promoter
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