载体基本信息 | |||
载体名称 | pDM276 | 载体抗性 | Ampicillin |
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载体长度 | 3518 bp | 载体类型 | Cloning vector |
载体来源 | Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ. |
pDM276 载体载体图谱
pDM276 载体载体序列
LOCUS Exported 3518 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pDM276, complete sequence. ACCESSION EU912548 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3518) AUTHORS Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ. TITLE A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum JOURNAL Plasmid 61 (2), 110-118 (2009) PUBMED 19063918 REFERENCE 2 (bases 1 to 3518) AUTHORS Veltman DM, Akar G, Bosgraaf L, van Haastert PJM. TITLE Direct Submission JOURNAL Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands REFERENCE 3 (bases 1 to 3518) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3518 /organism="Cloning vector pDM276" /mol_type="other DNA" /label=TAP-tag for C-terminal fusion /note="TAP-tag for C-terminal fusion" /db_xref="taxon:555769" misc_feature 10..87 /label=calmodulin binding peptide /note="calmodulin binding peptide" CDS 10..87 /codon_start=1 /product="calmodulin-binding peptide" /label=CBP /note="derived from skeletal muscle myosin light chain kinase; binds calmodulin with nanomolar affinity in the presence of calcium" /translation="KRRWKKNFIAVSAANRFKKISSSGAL" misc_feature 115..135 /label=Tobacco Etch Virus protease recognition sequence /note="Tobacco Etch Virus protease recognition sequence" CDS 115..135 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" misc_feature 175..534 /note="tandem repeat of Staphylococcus aureus protein A fragment" CDS 175..348 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL AEAKKLNDAQAPK" CDS 352..522 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA EAKKLNGAQAPK" primer_bind complement(579..595) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(632..650) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(671..687) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 695..711 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(719..749) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 764..785 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1017..1665) /direction=LEFT /label=pUC ori /note="pUC ori" rep_origin complement(1073..1661) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1832..2692) /codon_start=1 /product="penicillin beta-lactamase" /label=penicillin beta-lactamase /protein_id="ACH81575.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2693..2797) /gene="bla" /label=AmpR promoter rep_origin 2824..3279 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 3420..3436 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3446..3464 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 3490..3506 /label=KS primer /note="common sequencing primer, one of multiple similar variants"
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