载体基本信息 | |||
载体名称 | pDUAL-HFF21 | 载体抗性 | Ampicillin |
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载体长度 | 8770 bp | 载体类型 | S. pombe expression vector |
载体来源 | Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. |
pDUAL-HFF21 载体载体图谱
pDUAL-HFF21 载体载体序列
LOCUS Exported 8770 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION S. pombe expression vector pDUAL-HFF21 DNA, complete sequence. ACCESSION AB364119 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8770) AUTHORS Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. TITLE pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast JOURNAL Yeast 21 (15), 1289-1305 (2004) PUBMED 15546162 REFERENCE 2 (bases 1 to 8770) AUTHORS Matsuyama A, Shirai A, Yoshida M. TITLE A series of promoters for constitutive expression of heterologous genes in fission yeast JOURNAL Yeast 25 (5), 371-376 (2008) PUBMED 18437702 REFERENCE 3 (bases 1 to 8770) AUTHORS Matsuyama A, Shirai A, Yoshida M. TITLE Direct Submission JOURNAL Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL :http://cgl.riken.go.jp/ REFERENCE 4 (bases 1 to 8770) AUTHORS . TITLE Direct Submission COMMENT Fission yeast expression vector. FEATURES Location/Qualifiers source 1..8770 /organism="Schizosaccharomyces pombe expression vector pDUAL-HFF21" /mol_type="other DNA" /db_xref="taxon:477397" regulatory 1..237 /regulatory_class="terminator" /gene="ADH1" misc_feature 1..237 /gene="ADH1" /label=derived from Saccharomyces cerevisiae /note="derived from Saccharomyces cerevisiae" gene 1..237 /gene="ADH1" /label=ADH1 terminator 21..208 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" misc_feature 244..4768 /label=derived from Schizosaccharomyces pombe /note="derived from Schizosaccharomyces pombe" gene join(244..1470,4415..4768) /pseudo /gene="leu1" /label=leu1 regulatory 244..954 /regulatory_class="promoter" /pseudo /gene="leu1" CDS join(955..1470,4415..4768) /pseudo /gene="leu1" /label=leu1 regulatory complement(1483..1871) /regulatory_class="terminator" /gene="ura4" gene complement(1483..1871) /gene="ura4" /label=ura4 CDS complement(1872..2666) /codon_start=1 /gene="ura4" /product="orotidine 5'-phosphate decarboxylase" /label=ura4 /note="internal EcoRV site is eliminated." /protein_id="BAF90910.1" /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH" gene complement(1872..2666) /gene="ura4" /label=ura4 regulatory complement(2667..3190) /regulatory_class="promoter" /gene="ura4" gene complement(2667..3190) /gene="ura4" /label=ura4 rep_origin 3208..4400 /standard_name="ars1" /label=ars1 rep_origin 3275..4058 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" misc_feature 4769..7897 /label=derived from Cloning vector pUC119 /note="derived from Cloning vector pUC119" primer_bind complement(4781..4797) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5010..5465 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5747..5851 /gene="bla" /label=AmpR promoter CDS 5852..6712 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6883..7471 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7759..7780 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7795..7825 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7833..7849 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7857..7873 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" regulatory 7898..8636 /regulatory_class="promoter" /gene="tub1" misc_feature 7898..8636 /gene="tub1" /label=derived from Schizosaccharomyces pombe /note="derived from Schizosaccharomyces pombe" gene 7898..8636 /gene="tub1" /label=tub1 CDS 8662..8739 /codon_start=1 /product="hexahistidine and 2 copies of the FLAG epitope" /label=hexahistidine and 2 copies of the FLAG epitope /protein_id="BAF90911.1" /translation="MHHHHHHMDYKDDDDKPMDYKDDDDK" CDS 8665..8682 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 8686..8709 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 8716..8739 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK"
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