| 载体基本信息 | |||
| 载体名称 | pDUAL-HFF21 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 8770 bp | 载体类型 | S. pombe expression vector |
| 载体来源 | Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida M. | ||
pDUAL-HFF21 载体载体图谱
pDUAL-HFF21 载体载体序列
LOCUS Exported 8770 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION S. pombe expression vector pDUAL-HFF21 DNA, complete sequence.
ACCESSION AB364119
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8770)
AUTHORS Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida
M.
TITLE pDUAL, a multipurpose, multicopy vector capable of chromosomal
integration in fission yeast
JOURNAL Yeast 21 (15), 1289-1305 (2004)
PUBMED 15546162
REFERENCE 2 (bases 1 to 8770)
AUTHORS Matsuyama A, Shirai A, Yoshida M.
TITLE A series of promoters for constitutive expression of heterologous
genes in fission yeast
JOURNAL Yeast 25 (5), 371-376 (2008)
PUBMED 18437702
REFERENCE 3 (bases 1 to 8770)
AUTHORS Matsuyama A, Shirai A, Yoshida M.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical
Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
:http://cgl.riken.go.jp/
REFERENCE 4 (bases 1 to 8770)
AUTHORS .
TITLE Direct Submission
COMMENT Fission yeast expression vector.
FEATURES Location/Qualifiers
source 1..8770
/organism="Schizosaccharomyces pombe expression vector
pDUAL-HFF21"
/mol_type="other DNA"
/db_xref="taxon:477397"
regulatory 1..237
/regulatory_class="terminator"
/gene="ADH1"
misc_feature 1..237
/gene="ADH1"
/label=derived from Saccharomyces cerevisiae
/note="derived from Saccharomyces cerevisiae"
gene 1..237
/gene="ADH1"
/label=ADH1
terminator 21..208
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
misc_feature 244..4768
/label=derived from Schizosaccharomyces pombe
/note="derived from Schizosaccharomyces pombe"
gene join(244..1470,4415..4768)
/pseudo
/gene="leu1"
/label=leu1
regulatory 244..954
/regulatory_class="promoter"
/pseudo
/gene="leu1"
CDS join(955..1470,4415..4768)
/pseudo
/gene="leu1"
/label=leu1
regulatory complement(1483..1871)
/regulatory_class="terminator"
/gene="ura4"
gene complement(1483..1871)
/gene="ura4"
/label=ura4
CDS complement(1872..2666)
/codon_start=1
/gene="ura4"
/product="orotidine 5'-phosphate decarboxylase"
/label=ura4
/note="internal EcoRV site is eliminated."
/protein_id="BAF90910.1"
/translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
gene complement(1872..2666)
/gene="ura4"
/label=ura4
regulatory complement(2667..3190)
/regulatory_class="promoter"
/gene="ura4"
gene complement(2667..3190)
/gene="ura4"
/label=ura4
rep_origin 3208..4400
/standard_name="ars1"
/label=ars1
rep_origin 3275..4058
/label=ars1
/note="Schizosaccharomyces pombe autonomously replicating
sequence ars1"
misc_feature 4769..7897
/label=derived from Cloning vector pUC119
/note="derived from Cloning vector pUC119"
primer_bind complement(4781..4797)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 5010..5465
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5747..5851
/gene="bla"
/label=AmpR promoter
CDS 5852..6712
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6883..7471
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7759..7780
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7795..7825
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7833..7849
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7857..7873
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
regulatory 7898..8636
/regulatory_class="promoter"
/gene="tub1"
misc_feature 7898..8636
/gene="tub1"
/label=derived from Schizosaccharomyces pombe
/note="derived from Schizosaccharomyces pombe"
gene 7898..8636
/gene="tub1"
/label=tub1
CDS 8662..8739
/codon_start=1
/product="hexahistidine and 2 copies of the FLAG epitope"
/label=hexahistidine and 2 copies of the FLAG epitope
/protein_id="BAF90911.1"
/translation="MHHHHHHMDYKDDDDKPMDYKDDDDK"
CDS 8665..8682
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 8686..8709
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 8716..8739
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
我们不提供该载体，载体信息仅供查询和参考。